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73144

Sigma-Aldrich

Resorufin

95%

Sinonimo/i:

7-Hydroxy-3H-phenoxazin-3-one, NSC 12097

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About This Item

Formula empirica (notazione di Hill):
C12H7NO3
Numero CAS:
635-78-9
Peso molecolare:
213.19
Beilstein:
174850
Numero CE:
211-241-8
Numero MDL:
MFCD00128991
Codice UNSPSC:
12171500
ID PubChem:
329764655
NACRES:
NA.32

Livello qualitativo

100

Saggio

≥95.0% (HPLC)
95%

Forma fisica

solid

Punto di fusione

>300 °C (lit.)

Temperatura di conservazione

−20°C

Stringa SMILE

O=C(C=C1)C=C2C1=NC3=CC=C(O)C=C3O2

InChI

1S/C12H7NO3/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9/h1-6,14H
HSSLDCABUXLXKM-UHFFFAOYSA-N

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Categorie correlate

Descrizione generale

Red fluorophore standard. Widely used in enzyme assay systems. Absorption and fluorescence of resorufin are pH dependent. Below the pKa (~6.0), Abs. shifts to ~ 480 nm and both E and fluorescence quantum yield are markedly lower. Resorufin is unstable in the presence of thiols such as dithiothreitol (DTT) and 2-mercaptoethanol.
Resorufin has high extinction coefficient and good chemical and photostability. These properties of resorufin make it perfect for its usage in inverse fluorescence assay to detect H2O2 by measuring the disappearance of its fluorescence upon horseradish peroxidase-mediated oxidation to a nonfluorescent derivative.

Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H315,H319,H335

Classi di pericolo

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Sigma-Aldrich

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Lipase Substrate ≥95% (HPLC)

Sigma-Aldrich

30058

Lipase Substrate

A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases.
Zhou M, et al.
Analytical Biochemistry, 253(2), 162-168 (1997)
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Cytochrome P450 (P450) enzymes mediate arachidonic acid (AA) oxidation to several biologically active metabolites. Our aims in this study were to characterize AA metabolism by different recombinant rat P450 enzymes and to identify new targets for modulating P450-AA metabolism in
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