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72755

Sigma-Aldrich

5-(Bromomethyl)fluorescein

BioReagent, suitable for fluorescence, ≥95% (sum of tautomers, GC)

Sinonimo/i:

5-BMF

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About This Item

Formula empirica (notazione di Hill):
C21H13BrO5
Numero CAS:
148942-72-7
Peso molecolare:
425.23
Codice UNSPSC:
12352200
ID PubChem:
329764330

Nome Commerciale

BioReagent

Saggio

≥95% (sum of tautomers, GC)

Fluorescenza

λex 492 nm; λem 515 nm in 0.1 M Tris pH 9.0

Compatibilità

suitable for fluorescence

Stringa SMILE

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(CBr)ccc45)c1

InChI

1S/C21H13BrO5/c22-10-11-1-4-15-14(7-11)20(25)27-21(15)16-5-2-12(23)8-18(16)26-19-9-13(24)3-6-17(19)21/h1-9,23-24H,10H2
OQHKPJAZGYJYTB-UHFFFAOYSA-N

Confezionamento

Bottomless glass bottle. Contents are inside inserted fused cone.

Altre note

Labeling of nucleosides and nucleotides at the N-atom; labeling of carboxylic acids: shows very strong absorption and fluorescence.

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Olga Krystofova et al.
International journal of environmental research and public health, 10(1), 47-71 (2013-01-25)
Nanomaterials are structures whose exceptionality is based on their large surface, which is closely connected with reactivity and modification possibilities. Due to these properties nanomaterials are used in textile industry (antibacterial textiles with silver nanoparticles), electronics (high-resolution imaging, logical circuits
K J Baker et al.
Biochimica et biophysica acta, 1192(1), 53-60 (1994-06-01)
The Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum can be labelled at Cys-670 and Cys-674 with 5-[[2-[(iodoacetyl) amino]ethyl]amino]naphthalene-1-sulphonic acid (IAEDANS). Resonance energy transfer has been used to measure the distance between Cys-670/Cys-674 and Glu-439 labelled with 5-(bromomethyl)fluorescein as 40 A. The
H I Stefanova et al.
Biochemistry, 32(23), 6095-6103 (1993-06-15)
The (Ca(2+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum was labeled with 5-(bromomethyl)fluorescein. A stoichiometry of one label per ATPase molecule was found, which was unaffected by the presence of ATP. Labeling resulted in a 60% decrease in ATPase activity. Sequencing identified
U Alexiev et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(2), 372-376 (1995-01-17)
The pH-indicator dye fluorescein was covalently bound to the surface of the purple membrane at position 72 on the extracellular side of bacteriorhopsin and at positions 101, 105, 160, or 231 on the cytoplasmic side by reacting bromomethylfluorescein with the
P S Mukherjee et al.
Pharmaceutical research, 12(6), 930-936 (1995-06-01)
5-Bromomethyl fluorescein (5-BMF) was evaluated in this work as a pre-column, off-line derivatizing reagent for analytes containing a free carboxyl group. The reagent possessed high molar absorptivity and quantum yield and it's excitation maximum matched the intense 488.0 nm emission
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