60700

Nuclear Fast Red

for microscopy (Bot., Hist.)

• Sinonimo/i: 4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt
• Formula empirica (notazione di Hill): C₁₄H₈NNaO₇S
• Numero CAS: 6409-77-4
• Peso molecolare: 357.27
• Colour Index: 60760
• Numero CE: 229-088-0
• Numero MDL: MFCD00078493
• Codice UNSPSC: 12171500
• ID PubChem: 57651779
• NACRES: NA.47

Proprietà

• Grado: for microscopy (Bot., Hist.)
• Forma fisica: powder
• Solubilità: methanol: water (1:1): 0.01 g/10 mL, red
• εmax: ≥275 at 550-560 nm in DMSO; ≥300 at 516-526 nm in DMSO
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: room temp
• Stringa SMILE: [Na+].Nc1c(O)c(c(O)c2C(=O)c3ccccc3C(=O)c12)S([O-])(=O)=O
• InChI: 1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1; IFSXZLJQEKGQAF-UHFFFAOYSA-M

Descrizione

Descrizione generale

Nuclear Fast Red (C.l. 60760) is a substituted hydroxy anthraquinone chromophore that can chelate metal ions. It is also known as kernechtrot and 4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt. It is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is a dry staining dye that makes target structures (by fixing, wherenecessary embedding, staining with the above nuclear fast red solution, counterstaining, mounting) in human-histological specimen materials, for examplehistological sections of e. g. the kidney, evaluable for diagnostic purposes.

Applicazioni

Nuclear fast red has been used for the nuclear staining of PB (Prussian blue) positive cells in the rostral sub-epidermis and upper beak coronal region isolated from pigeons.Recent applications include:

• staining sperm and spermatids in semen
• identifying areas for PCR analysis in paraffin-embedded tissues
• nuclear counterstaining following immunostaining and silver staining

Used as a biological stain

Azioni biochim/fisiol

Nuclear fast red dye has anthraquinonic nature and is used either alone or in combination with other dyes. It is mainly used for staining of nuclei.

Principio

Nuclear fast red can be used for simple nuclear staining. It is an acidic dyebelonging to the group of anthraquinone dyes. Aluminum sulfate, functioning as a mordant, is added to form a color lake that enables red nuclear staining. Nuclear fast red-aluminum sulfate is used for counterstaining in histochemicalreactions, e. g. in Berlin blue staining for detection of iron or in silver staining. Nuclear fast red for microscopy Certistain^® can also be used fornuclear staining with alcian blue.

Note legali

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves