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53608

Biotin (5-fluorescein) conjugate

BioReagent, suitable for fluorescence, ≥90% (HPCE)

Sinonimo/i:

N-(Biotinylamidoethyl)-fluorescein-5-carboxamide, N-(Fluorescein-5-carbamidoethyl)biotinamide

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Cambia visualizzazione
Voi/SKUDisponibilitàPrezzo
1 mg
Per conoscere la disponibilità, visualizza il carrello
CHF 171.00
5 mg
Per conoscere la disponibilità, visualizza il carrello
CHF 640.00

Informazioni su questo articolo

Formula empirica (notazione di Hill):
C33H32N4O8S
Numero CAS:
957494-27-8
Peso molecolare:
644.69
UNSPSC Code:
12352108
eCl@ss:
34058011
PubChem Substance ID:
57651424
NACRES:
NA.32
MDL number:
MFCD03791084

CHF 171.00

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product line

BioReagent

Quality Segment

assay

≥90% (HPCE)

solubility

DMSO: soluble

fluorescence

λex 490 nm; λem 524 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)NCCNC(=O)c3ccc(c(c3)C(O)=O)C4=C5C=CC(=O)C=C5Oc6cc(O)ccc46)[C@@]1([H])NC(=O)N2

InChI

1S/C33H32N4O8S/c38-18-6-9-21-25(14-18)45-26-15-19(39)7-10-22(26)29(21)20-8-5-17(13-23(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30-/m0/s1

InChI key

DFUFXKZUEOKPSD-LFERIPGTSA-N

Application

Biotin (5-fluorescein) conjugate is a reagent that may be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Biotin-fluorescein conjugate for the fluorometric, accurate measurement of avidin and streptavidin.
Often referred to as Biotin (4-fluorescein) conjugate.

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Questo articolo
B9431343222189727
Biotin (5-fluorescein) conjugate BioReagent, suitable for fluorescence, ≥90% (HPCE)

Sigma-Aldrich

53608

Biotin (5-fluorescein) conjugate

Biotin-4-Fluorescein ≥95.0% (HPLC)

Sigma-Aldrich

B9431

Biotin-4-Fluorescein

Biotin, Fluorescein Conjugate

Sigma-Aldrich

343222

Biotin, Fluorescein Conjugate

Avidin, Fluorescein Conjugate This Avidin, Fluorescein Conjugate is validated for use in Immunohistochemistry, Immunfluorescence.

Sigma-Aldrich

189727

Avidin, Fluorescein Conjugate

description

BioReagent, suitable for fluorescence, ≥90% (HPCE)

description

≥95.0% (HPLC)

description

-

description

This Avidin, Fluorescein Conjugate is validated for use in Immunohistochemistry, Immunfluorescence.

suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

-

fluorescence

λex 490 nm; λem 524 nm in 0.1 M Tris pH 8.0

fluorescence

-

fluorescence

-

fluorescence

-

solubility

DMSO: soluble

solubility

-

solubility

DMF: 5 mg/mL, DMSO: 5 mg/mL, methanol: 5 mg/mL

solubility

-

assay

≥90% (HPCE)

assay

≥95.0% (HPLC)

assay

-

assay

-

Quality Level

100

Quality Level

200, 100

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

room temp

storage temp.

−20°C

storage temp.

−20°C

Classe di stoccaggio

11 - Combustible Solids

Che succede?

WGK 3

Punto di infiammabilità (°F)

Not applicable

Punto di infiammabilità (°C)

Not applicable

DPI (dispositivi di protezione individuale)

Eyeshields, Gloves, type N95 (US)

    • Scegli una delle versioni più recenti:

    Certificati d'analisi (COA)

    Lot/Batch Number
    BCCN9853
    BCBH5840V
    BCCL5272
    BCBR8713V
    BCBW4689

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    Questions

    1–3 of 3 Questions  

    1. What is the recommended concentration for dilution?

      1 answer

      1. The solubility of Biotin (5-fluorescein) conjugate 53608-1MG-F is not a specification for product release.
        However it should be soluble in DMSO (D8418) at least up to a concentration of 3 mM.
        The concentration for dilution will be dependent on the experiment design and will need to be determined empirically unless you have an academic paper similar to your experiment design that provides that information.

        The following citation may be of interest for protocol reference.

        J Vis Exp. 2020 May 13;(159). doi: 10.3791/61146.
        Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
        PMID: 32478756
        https://pubmed.ncbi.nlm.nih.gov/32478756/

        Helpful?

    2. What is the extinction coefficient for product 53608.

      1 answer

      1. Although the excitation and emission wavelengths are measured during quality control testing, there is no testing to determine the extinction coefficient for 53608 (Biotin (5-fluorescein) conjugate). There was no information on file regarding the extinction coefficient for product 53608.

        Helpful?

    3. what are the solvents required to dilute these two reagents and also the recommended concentration?

      1 answer

      1. The solubility of Biotin (5-fluorescein) conjugate 53608-1MG-F is not a specification for product release, but it should be soluble in DMSO at least up to a concentration of 3 mM.

        Helpful?

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