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Key Documents

52944

Sigma-Aldrich

Anti-Rabbit IgG−Abberior® STAR 488 antibody produced in goat

for STED application

Sinonimo/i:

Abberior® STAR 488-Anti-Rabbit IgG antibody produced in goat

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.32

Origine biologica

goat

Livello qualitativo

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

rabbit

Disponibilità

available only in USA and Canada

Concentrazione

~1 mg/mL

Fluorescenza

λex 503 nm; λem 524 nm in PBS, pH 7.4

Temperatura di conservazione

−20°C

Descrizione generale

Abberior STAR 488 was developed for STED and confocal microscopy in the green spectral region. It is a bright green fluorescent dye. The dye works extremely well with Abberior Instruments Expert Line Microscopes and Leica STED microscopes. It can be very effectively excited with the prominent 488 nm laser line. For STED microscopy, Abberior STAR 488 can be efficiently used with STED Laser wavelengths between 590 - 610 nm. Abberior STAR 488 can substitute dyes like Oregon Green 488, Atto 488 or Alexa Fluor 488. Abberior STAR 488 is the ideal partner for Abberior STAR 440SXP to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.

Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 503 (PBS pH 7.4), 504 (H2O), 507 (MeOH + 0.1% TFA)
Extinction Coefficient, εmax [M-1cm-1]: 65 000 (PBS pH 7.4), 80 000 (H2O), 85 000 (MeOH + 0.1% TFA)
Correction Factor, CF260 = ε260max: 0,28 (PBS pH 7.4)
Correction Factor, CF280 = ε280max: 0,14 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 524 (PBS pH 7.4), 525 (H2O), 531 (MeOH + 0.1% TFA)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,89 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,9 (PBS pH 7.4)

Applicazioni

Anti-Rabbit IgG-Abberior® STAR 488 antibody has been used as a secondary antibody:
  • for fluorescent immunohistochemistry in prostate cancer cell lines, PPC-1 and TSU-Pr1
  • for immunofluorescence in HeLa and monkey kidney COS-7 cells
  • for STED (stimulated emission depletion) microscopy in OLN-t40-α-syn cells (oligodendroglial cells)

Compatibilità

Designed and tested for fluorescent super-resolution microscopy

Risultati analitici

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Note legali

ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Katharina Pukaß et al.
Frontiers in cellular neuroscience, 9, 163-163 (2015-05-23)
α-Synuclein (α-syn) positive glial cytoplasmic inclusions (GCI) originating in oligodendrocytes (ODC) are a characteristic hallmark in multiple system atrophy (MSA). Their occurrence may be linked to a failure of the ubiquitin proteasome system (UPS) or the autophagic pathway. For proteasomal
Giuseppe Vicidomini et al.
Methods (San Diego, Calif.), 66(2), 124-130 (2013-07-03)
Stimulation emission depletion (STED) microscopy breaks the spatial resolution limit of conventional light microscopy while retaining its major advantages, such as working under physiological conditions. These properties make STED microscopy a perfect tool for investigating dynamic sub-cellular processes in living
Combination of axitinib and dasatinib for anti-cancer activities in two prostate cancer cell lines.
Peng N
Bangladesh Journal of Pharmacology, 11, 10-10 (2016)
T A Klar et al.
Optics letters, 24(14), 954-956 (2007-12-13)
We overcame the resolution limit of scanning far-field fluorescence microscopy by disabling the fluorescence from the outer part of the focal spot. Whereas a near-UV pulse generates a diffraction-limited distribution of excited molecules, a spatially offset pulse quenches the excited
Tim Grotjohann et al.
Nature, 478(7368), 204-208 (2011-09-13)
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported

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