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5086

Sigma-Aldrich

CD2 human

recombinant, expressed in E. coli, 0.5 mg protein/mL

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.75

Origine biologica

human

Ricombinante

expressed in E. coli

Descrizione

0.1 mg recombinant human CD2 in 20 mM Tris-HCl buffer, pH 8.0, containing NaCl, KCl, EDTA, L-arginine, DTT, and glycerol

Sterilità

Filtered sterilized solution

Saggio

≥90% (SDS-PAGE)

Forma fisica

liquid

Confezionamento

pkg of 100 μg

Concentrazione

0.5 mg protein/mL

tecniche

cell culture | mammalian: suitable

Numero d’accesso

NP_001758

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... CD2(914)

Applicazioni

Coating a plate well (6 well plate) with this recombinant CD2 protein in a T cell specific medium at 1-10 μg/well allows for human T cell differentiation studies in vitro.

Use this procedure as a guideline to determine optimal coating conditions for the culture system of choice.
1. Thaw CD2 and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently dilute so that the volume added covers the surface evenly.
2. Add appropriate amount of diluted material to culture surface.
3. Incubate at room temperature for approximately 1.5 hours.
4. Aspirate remaining material.
5. Rinse plates carefully with water and avoid scratching bottom surface of plates.
6. Plates are ready for use. They may also be stored at 2-8 °C damp or air dried if sterility is maintained.



Sequenza

MASMTGGQQMGRGHHHHHHGNLYFQGGEFELKEITNALETWGALGQDINLDIPSFQMSDDIDDIKWEKTSDKKKIAQFRKEKETFKEKDTYKLFKNGTLKIKHLKTDDQDIYKVSIYDTKGKNVLEKIFDLKIQERVSKPKISWTCINTTLTCEVMNGTDPELNLYQDGKHLKLSQRVITHKWTTSLSAKFKCTAGNKVSKESSVEPVSCPEKGLD

Nota sulla preparazione

The full-length extracellular domain of the human CD2 gene (25-209 aa) was constructed with 31 N-terminal T7/HIS-tag and expressed in E. coli as inclusion bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Clare M Baecher-Allan et al.
Journal of immunology (Baltimore, Md. : 1950), 186(6), 3317-3326 (2011-02-09)
Studying the activity of homogeneous regulatory T cell (Treg) populations will advance our understanding of their mechanisms of action and their role in human disease. Although isolating human Tregs exhibiting low expression of CD127 markedly increases purity, the resulting Treg
B Schraven et al.
Nature, 345(6270), 71-74 (1990-05-03)
At least two membrane receptors have been defined through which human T lymphocytes can be induced to proliferate and differentiate, namely the CD3-Ti antigen receptor complex and the CD2 molecule. Monoclonal antibodies directed at either CD2 or CD3 induce intracellular
Eun-Ok Kim et al.
The Journal of biological chemistry, 285(53), 41755-41764 (2010-09-04)
Human natural killer (NK) cells express an abundant level of 2B4 and CD2 on their surface. Their counter-receptors, CD48 and CD58, are also expressed on the NK cell surface, raising a question about the functional consequences of potential 2B4/CD48 and

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