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3011

Sigma-Aldrich

Transcreener® ADP2 TR-FRET Red Assay

Sinonimo/i:

Transcreener assay

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Spedizione prevista il16 aprile 2025

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About This Item

Codice UNSPSC:
41122100
NACRES:
NA.84

CHF 929.00

Spedizione prevista il16 aprile 2025

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

The Transcreener® ADP2 TR-FRET Red Assay is a competitive immunoassay for ADP with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. Because it is highly selective for ADP, the assay can be used with any enzyme that converts ATP to ADP, regardless of what other substrates are used. Examples of enzymes include protein, lipid, and carbohydrate kinases, ATPases, DNA helicases, carboxylases, and glutamine synthetase.
The Transcreener® assay is designed specifically for high-throughput screening (HTS), with a single addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener® HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families.

The Transcreener® ADP2 TR-FRET Red Assay provides the following benefits:
  • Accommodates ATP concentrations ranging from 0.1 μM to 1,000 μM. • Excellent data quality (Z′ = 0.7) at low substrate conversion (typically 10% or less).
  • Overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by using a single set of assay reagents that detect an invariant product.
  • Time-gated detection method largely eliminates interference that can result from prompt fluorescence of test compounds.
  • Far-red tracer further minimizes interference from fluorescent compounds and light scattering.

View full Transcreener® product list

Quantità

3011-1K = 1,000 assay, 384-well
3011-10K = 10,000 assay, 384-well

Stato fisico

Kit with buffered aqueous solutions

Note legali

Transcreener is a registered trademark of BellBrook Labs

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Certificati d'analisi (COA)

Lot/Batch Number
13642

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D N Angelov et al.
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