19899

CHAPS 100 mM solution

• Sinonimo/i: CHAPS solution
• Formula empirica (notazione di Hill): C₃₂H₅₈N₂O₇S
• Numero CAS: 75621-03-3
• Peso molecolare: 614.88
• Numero MDL: MFCD00012116
• Codice UNSPSC: 12161902
• ID PubChem: 57647807
• NACRES: NA.25

Proprietà

• Descrizione: zwitterionic
• Forma fisica: liquid
• PM: 614.88 g/mol
• Concentrazione: 100 mM in H₂O
• tecniche: protein quantification: suitable
• CMC: 8 mM
• Temp. transizione: cloud point >100 °C
• Densità: 1.01 g/mL at 20 °C
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1; UMCMPZBLKLEWAF-RFCNGIAKSA-N

Descrizione

Descrizione generale

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) is a biocompatible surfactant. It is a derivative of cholic acid comprising a hydrophobic cholesterol-like skeleton possessing three hydroxyl groups and a zwitterionic aliphatic tail.

Applicazioni

CHAPS 100 mM solution has been used as a component of lysis buffer for the extraction of proteins from goat endometrial epithelial cells (EECs) and liver tissue.

Azioni biochim/fisiol

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) exhibits the properties of both sulfobetaine-type surfactants and bile salts. It is involved in the protein extraction process for proteomic analysis and reconstitution of membrane proteins into lipid vesicles (proteoliposomes). CHAPS is broadly used in biochemistry for membrane protein and receptor isolation and purification. It is conveniently used in pharmaceuticals and medicines due to its biocompatibility, noncharged character, nondenaturing, and disaggregating properties.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves