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Merck
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Documenti

102-05A

Sigma-Aldrich

Human Epidermal Keratinocytes: HEK, adult

Sinonimo/i:

HEK cells

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About This Item

Codice UNSPSC:
41106514
NACRES:
NA.81

Origine biologica

human neonatal foreskin or adult skin (normal)

Livello qualitativo

Confezionamento

pkg of 500,000 cells

Produttore/marchio commerciale

Cell Applications, Inc

Modalità di accrescimento

Adherent

Cariotipo

2n = 46

Morfologia

Epidermal

tecniche

cell culture | mammalian: suitable

Malattie correlate

allergies; cancer

Condizioni di spedizione

dry ice

Temperatura di conservazione

−196°C

Categorie correlate

Descrizione generale

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Epidermal keratinocytes comprise 90 % of all cells found in the epidermis, the outermost layer of skin. They produce keratin, the intermediate filament, largely responsible for skin’s barrier function. Through their interactions with epidermal melanocytes, keratinocytes obtain melanin and store it as a means of protection from UV radiation.

HEK provide an excellent model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology, as well as HPV infection.

When grown on inserts and provided with the liquid/air interface, HEK can differentiate into a stratified squamous epithelium and serve as a more physiological 3D tissue model for in vitro studies.

Origine della linea cellulare

Skin

Applicazioni

Skin biology and toxicology, wound healing, artificial skin, cell proliferation, migration, UV light response

Componenti

Basal Medium containing 10% FBS & 10% DMSO

Nota sulla preparazione

  • Primary culture, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 16 doublings

Mantenimento delle subcolture

Please refer to the HEK Culture Protocol.

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Shunfeng Wang et al.
Molecules (Basel, Switzerland), 25(21) (2020-11-14)
In the present study, the fabrication of a biomimetic wound dressing that mimics the extracellular matrix, consisting of a hydrogel matrix composed of non-oxidized and periodate-oxidized marine alginate, was prepared to which gelatin was bound via Schiff base formation. Into

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