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05019

7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin

BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

Sinonimo/i:

MDCC

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Voi/SKUDisponibilitàPrezzo
5 mg
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CHF 163.00

Informazioni su questo articolo

Formula empirica (notazione di Hill):
C20H21N3O5
Numero CAS:
Peso molecolare:
383.40
UNSPSC Code:
12352116
PubChem Substance ID:
NACRES:
NA.32
MDL number:

CHF 163.00


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product line

BioReagent

Quality Segment

assay

≥97.0% (HPLC)

suitability

suitable for fluorescence

SMILES string

CCN(CC)c1ccc2C=C(C(=O)NCCN3C(=O)C=CC3=O)C(=O)Oc2c1

InChI

1S/C20H21N3O5/c1-3-22(4-2)14-6-5-13-11-15(20(27)28-16(13)12-14)19(26)21-9-10-23-17(24)7-8-18(23)25/h5-8,11-12H,3-4,9-10H2,1-2H3,(H,21,26)

InChI key

IXQPRUQVJIJUEB-UHFFFAOYSA-N

Application

Thiol-reactive probe for protein labelling
7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin is utilized as a fluoresencent biological sensing device . Used for real-time measurements for the release of inorganic phosphates during enzymatic reaction when MDCC is conjugated to a mutant phosphate-binding protein . Also, utilized for intramolecular fluorescence energy transfer (FRET) experiments .

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Questo articolo
G762700062107CB_05071502
MDCK-II Cell Line canine NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

00062107

MDCK-II Cell Line canine

vibrant-m

MDCK-SIAT1 Cell Line canine

description

BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

description

≥90%

description

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

description

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 5071502, canine kidney, Epithelial

suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

-

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

-

assay

≥97.0% (HPLC)

assay

≥90%

assay

-

assay

-

product line

BioReagent

product line

-

product line

-

product line

-

high-purity 7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin

-

-

G418 resistant MDCK-SIAT1 cells, MDCK-SIAT1 cell line, MDCK-SIAT1 mammalian cell culture, MDCK-SIAT1 cells with sialic acid overexpression, 10% FBS MDCK-SIAT1 cell line, canine cell line for cell culture applications, MDCK-SIAT1 cells for drug testing, MDCK-SIAT1 cells for sale, DMEM-based MDCK-SIAT1 cell culture, immortalized canine cell line for research, canine Cocker Spaniel kidney cell line, mammalian cell line with glutamine supplementation, canine kidney cell line for transfection


pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves



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Questions

  1. What do the excitation/emission peaks of this dye look like?

    1 answer
    1. The product specification requires an emission wavelength range of 460-470nm. The product is not screened for excitation wavelength, however it is expected to fall within a range of 416 to 425 nm. The compound is typically used for real-time measurements for the release of inorganic phosphates during enzymatic reaction when MDCC is conjugated to a mutant phosphate-binding protein . Please see the link below to review a publication that may be of interest:
      https://pubs.acs.org/doi/10.1021/bi9804277

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