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03978

Sigma-Aldrich

Methylene Blue solution

for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

Sinonimo/i:

Loeffler’s Methylene Blue

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250 ML
CHF 53.00

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Cambia visualizzazione
250 ML
CHF 53.00

About This Item

Formula empirica (notazione di Hill):
C16H18ClN3S
Numero CAS:
Peso molecolare:
319.85
Beilstein:
3641570
Numero MDL:
Codice UNSPSC:
12171500
ID PubChem:
NACRES:
NA.47

CHF 53.00


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Grado

for microscopy

Stato

liquid

Qualità

concentrated, aqueous solution

Durata

~3 yr

Concentrazione

1.5% (1.5 g/ 100 mL)

Densità

1.0 g/mL at 20 °C

εmax

9.4 at 656-666 nm in water

Compatibilità

suitable for microscopy

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
CXKWCBBOMKCUKX-UHFFFAOYSA-M

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Descrizione generale

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.
Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye.[1] It is a small molecular weight dye,[2] which is effective against Gram positive and Gram negative bacteria.[3]

Applicazioni

Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability.[4] It has also been used to stain sections to determine the presence of mycobacteria.[5][6]
Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Questions

  1. Concerning the stated concentration of the product 03978 (Methylene blue solution, 1.5%), what is the uncertainty and lot-to-lot variability?

    1 answer
    1. Product 03978 is not a GMP quality product. No testing is performed during Quality Control testing that measures lot to lot variability. The testing includes tests for Appearance, Thin Layer Chromatography, Density, Wavelength 1 at 656-666nm, Extinction Coefficient for Wavelength 1 and the solvent used to measure the wavelength and extinction coefficient. The information about a 1.5% concentration of dye is from the production documents used to manufacture the dye solution. The actual dye concentration is not measured.

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