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RNAINH

Sigma-Aldrich

Murine RNase Inhibitor

suitable for cDNA synthesis, suitable for RT-PCR

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About This Item

Codice UNSPSC:
41106300
NACRES:
NA.54

Forma fisica

liquid

Livello qualitativo

Concentrazione

40 unit/μL

tecniche

PCR: suitable
RT-PCR: suitable
cDNA synthesis: suitable
qPCR: suitable

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Descrizione generale

Murine RNase Inhibitor is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form that can extensively inhibit various RNases (RNase A, B, C).
Compared with human-derived RNase inhibitors, mouse-derived RNase inhibitors do not contain two cysteines that are very sensitive to oxidation in human protein, so it has higher antioxidant activity and is more suitable for experiments sensitive to high DTT (such as qPCR).
This product is DNase, RNase and Nickase free.

Applicazioni

Murine RNase Inhibitor is Suitable for use in:
  • in vitro inhibition of ribonucleases.
  • cDNA synthesis.
  • RT-PCR.
  • in vitro transcription and translation.

Caratteristiche e vantaggi

Murine RNase Inhibitor has the following advantages:

RT-PCR/qPCR Compatibility:
  • Enhanced antioxidant capacity will not form the disulfide bonds that human-derived proteins would produce under oxidative conditions.
  • High-purity E. coli soluble expression.
  • No RNase residue.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg


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