Saline-Sodium Phosphate-EDTA is used as a hybridization buffer in Northern and Southern blotting. It is also used as a wash buffer in DNA-microarray technique.
Caratteristiche e vantaggi
Ready-to-use buffer
No measuring and pH adjusting needed
Save time and minimize effort
Biological tests: free of DNase, RNase, protease, and nickase
Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
Confezionamento
Foil pouches
Ricostituzione
Contents of one pouch, when dissolved in 1000 mL of ultrapure water, will yield a 1× solution containing 150 mM NaCl, 10 mM NaH2PO4, 1 mM EDTA, pH 7.4 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.
Stoccaggio e stabilità
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Altre note
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Note legali
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
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