E4884

Acido etilendiamminotetraacetico

ACS reagent, 99.0-101.0%

• Sinonimo/i: EDTA, EDTA-Na₂, Edatamil, Edetato, Etilendiamminotetraacetato di disodio, Sequestrene Na₂
• Formula empirica (notazione di Hill): C₁₀H₁₄N₂Na₂O₈ · 2H₂O
• Numero CAS: 6381-92-6
• Peso molecolare: 372.24
• Beilstein: 3900609
• Numero CE: 205-358-3
• Codice UNSPSC: 12352107
• ID PubChem: 24894586
• NACRES: NA.77

Proprietà

• Grado: ACS reagent
• Livello qualitativo: 200
• agenzia: suitable for SM 4500 - NH3
• Saggio: 99.0-101.0%
• Forma fisica: solid
• Impiego in reazioni chimiche: reagent type: chelator
• Impurezze: ≤0.005% Insoluble matter; ≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)
• pH: 4.0-6.0 (25 °C, 5%)
• Punto di fusione: 248 °C (dec.) (lit.)
• Solubilità: H₂O: 100 g/L at 20 °C
• Cationi in tracce: Fe: ≤0.01%; heavy metals: ≤0.005% (by ICP-OES)
• applicazioni: microbiology
• Temperatura di conservazione: room temp
• Stringa SMILE: O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2; OVBJJZOQPCKUOR-UHFFFAOYSA-L

Descrizione

Descrizione generale

Ethylenediaminetetraacetic acid disodium salt dihydrate or EDTA, is a chelating agent with many applications in molecular biology. EDTA is often used in buffers as a metal ion inhibitor to maintain the stability and integrity of biological samples. EDTA binds to metal cations by forming a stable octahedral complex. Its ability to chelate metal ions makes it extremely useful for blocking metal-dependent enzymatic activities, such as DNases and proteases. Additionally, EDTA is often found in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Applicazioni

Antioxidant assays (1) EDTA is also employed in molecular biology techniques, such as DNA extraction, purification, and electrophoresis, where it helps to prevent metal-mediated degradation of nucleic acids. Additionally, EDTA is utilized in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Ethylenediaminetetraacetic acid disodium salt dehydrate has been used as a component:

• of radioimmunoprecipitation assay buffer (RIPA buffer) for preparation of C2C12 mouse skeletal myoblasts whole cell lysate
• of lysis buffer and IgG resin wash and equilibration buffer for the purification of human proteasome regulatory particles (RPs)
• of M-buffer for the isolation of human cardiomyocyte progenitor cells (CMPCs) from fetal heart

Chelante di cationi bivalenti. Inibisce enzimi, come le metalloproteasi, che per essere attivi necessitano di cationi bivalenti.

Caratteristiche e vantaggi

American Chemical Society (ACS) grade is suitable for use in standard analytical methods

Altre note

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Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H332,H373
• Consigli di prudenza: P260 - P271 - P304 + P340 + P312 - P314 - P501
• Classi di pericolo: Acute Tox. 4 Inhalation - STOT RE 2 Inhalation
• Organi bersaglio: Respiratory Tract
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)