UC0E03

UCOE^® Dual Expression Puromycin Vector Set

• Sinonimo/i: UCOE Mammalian Protein Expression Vectors, UCOE Mammalian Protein Expression Plasmids, Ubiquitous Chromatin Opening Element, UCOE03
• Codice UNSPSC: 41106621
• eCl@ss: 34360190
• NACRES: NA.51

Proprietà

• Condizioni di spedizione: dry ice
• Livello qualitativo: 100

Descrizione

Descrizione generale

The Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing.

CET 1019 HD-puro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.

CET 1019 AD-puro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.

The CET 1019 HD-puro-SceI and CET 1019 AD-puro-SceI vectors possess the following features:

• A multiple cloning site downstream of the UCOE-CMV combinations containing unique restriction enzyme sites to permit easy insertion of any gene of interest (cDNA or genomic)
• A polyadenylation element from the SV40 early region located downstream of the multiple cloning site for appropriate mRNA 3 end formation
• A puromycin antibiotic resistance gene for selection of stably transfected mammalian cells
• A β-lactamase gene for plasmid selection and propagation in transformed prokaryotic cells in the presence of ampicillin
• An I-SceI homing restriction endonuclease site for linearization of constructs prior to transfection into mammalian cells, which favors integration into the target cell genome via the pre-generated free DNA ends and thus retains the integrity of the UCOE-CMV-transgene cassettes

THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT licensing@emdmillipore.com.

Componenti

1) 10ug CET 1019 HD-puro-SceI (+) Vector (CS221292)

2) 10ug CET 1019 AD-puro-SceI (+) Vector (CS221293))

3) 10ug DC HD-puro (-) Vector (CS221283)

4) 10ug DC AD-puro (-) Vector (CS221295)

Altre note

Concentration: Please refer to lot specific datasheet.

Note legali

UCOE is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids