SCC428

D4M-3A Mouse Melanoma Cell Line

• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: mouse
• Growth mode: N/A
• Technique(s): cell culture | mammalian: suitable

Proprietà

• biological source: mouse
• Quality Segment: 100
• packaging: vial of >1X10⁶ cells
• manufacturer/tradename: Millipore
• growth mode: N/A
• technique(s): cell culture | mammalian: suitable
• shipped in: liquid nitrogen
• storage temp.: −196°C

Descrizione

Application

• Each vial contains > 1X106 viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, human, chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.

Melanoma is one of the most prevalent human cancers, affecting 1 out of every 50 individuals in their lifetime.1 The BRafV600E mutation, which occurs in nearly 50% in human melanomas, constitutively activates pERK, a component of the MAP kinase signaling pathway, and contributes to disease progression.2 Although human melanoma cell lines harboring the BRafV600E mutation are well-established, the reliance on xenografts in immunocompromised mice limits studies of host-tumor cell interactions in a syngeneic background.2
The D4M-3A cell line is a melanoma cell line from the conditional mouse model of metastatic melanoma: Tyr::CreER, BrafCA;Ptenlox/lox, which recapitulates human disease. Cultured D4M-3A cells express high constitutive pERK and respond to the BRafV600E inhibitor, Vemurafenib (PLX4032).2 D4M-3A cells lack melanin expression but maintain ability to express melanocyte differentiation antigens, which are important immune recognition factors. D4M-3A cells are transplantable in either immune-compromised or syngeneic B6 mice and show correlation of in vitro studies on molecular mechanisms of melanoma with in vivo investigations on pathology and immunology. The D4M-3A cell line is a clinically relevant model for both in vitro and in vivo studies of metastatic melanoma.

Source
The D4M-3A cell line was derived from a male transgenic Braf/Pten mouse.2

References
1. CA Cancer J Clin 2004, 54(3): 131-149.
2. Pigma Cell Melanoma Res 2014, 27(3): 495-501

Features and Benefits

The D4M-3A cell line is a clinically relevant model for both in vitro and in vivo studies of metastatic melanoma

Preparation Note

The cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting functionality.

Other Notes

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact [email protected].

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.;<bold>This product contains genetically modified organisms (GMO). </bold>Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges to request certain information about you and the establishment where the GMOs are being handled. <IS7_ContentPageCategoryLink target="Up.b.qB.CN8AAAFAnd8QWRbW">Click here for Enduser Declaration (EUD) Form</IS7_ContentPageCategoryLink>.

Informazioni sulla sicurezza

• Classe di stoccaggio: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable