PS1089

PhosphoDetect Anti-RhoA (pSer¹⁸⁸) Rabbit pAb

liquid, Calbiochem^®

• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: liquid
• contiene: ≤0.1% sodium azide as preservative
• Reattività contro le specie: human
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Isotipo: IgG
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: phosphorylation (pSer188)
• Informazioni sul gene: human ... RHOA(387)

Descrizione

Descrizione generale

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~22 kDa RhoA protein when expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.

Recognizes the ~22 kDa RhoA protein, expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.

This PhosphoDetect Anti-RhoA (pSer¹⁸⁸) Rabbit pAb is validated for use in Immunoblotting, ELISA for the detection of RhoA (pSer¹⁸⁸).

Immunogeno

Human

a synthetic peptide corresponding to amino acids surrounding the Ser¹⁸⁸ phosphorylation site of human RhoA, coupled to KLH

Applicazioni

Immunoblotting (1:1000)
ELISA (1:2000)

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In PBS, 1 mg/ml BSA, 50% glycerol.

Ricostituzione

Do not aliquot.

Risultati analitici

Positive Control
RhoA GST fusion protein

Altre note

Does not recognize unphosphorylated RhoA. The immunogen sequence is conserved in rat and mouse RhoA, but cross-reactivity has not been tested. The immunogen sequence is not found in the RhoB and RhoC sequences. For immunoblotting, the membrane was incubated with antibody diluted in PBS/5% nonfat milk/0.04% Tween^® 20 detergent for 1 h at room temperature. Antibody should be titrated for optimal results in individual systems.

Ellerbroek, S.M., et al. 2003. J. Biol. Chem.278, 19023.
Sawada, N., et al. 2001. Biochem. Biophys. Res. Commun. 280, 798.
Dong, J.M., et al. 1998. J. Biol. Chem.273, 22554.
Lang, P., et al. 1996. EMBO J.15, 510.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1