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Documenti fondamentali

PC67

Sigma-Aldrich

Anti-Bcl-x L (Ab-1) (201-216) Rabbit pAb

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About This Item

Forma dell’anticorpo

purified antibody

Livello qualitativo

Clone

polyclonal

Stato

liquid

contiene

≤0.1% sodium azide as preservative

Reattività contro le specie

rat, human, opossum, mouse

Isotipo

IgG

Descrizione generale

Purified rabbit polyclonal antibody. Recognizes the ~28 kDa Bcl-x protein.
Recognizes the ~28 kDa Bcl-x protein in doxorubicin-treated MCF-7 cells.
bcl-x is a bcl-2-related gene that can function as a regulator of programmed cell death (apoptosis) independent of bcl-2. Alternative splicing results in two distinct bcl-x mRNAs, bcl-xL and bcl-xS. The larger mRNA gives rise to a protein product, bcl-xL, which is similar in size and predicted structure to bcl-2. bcl-x immunoreactivity has been detected in a wide variety of cell types and the protein is typically present in the cytosol in association with the mitochondrial periphery, a property shared with bcl-2. Of the two isoforms of bcl-x, the long (bcl-xL) is the most abundant mRNA species expressed in embryonic and adult tissues and most likely differs from bcl-2 in its regulatory activity on cell differentiation through controlled tissue specific expression. Like its homolog bcl-2, the mechanism and extent of bcl-x′s function are still increasing. bcl-xL has been shown to modify the cell′s response to oxidants as well as to participate in resistance to chemotherapeutic agents and radiation.

Immunogeno

a synthetic peptide (PWIQENGGWDTFVELY) corresponding to amino acids 201-216 of human Bcl-x

Applicazioni

Frozen Sections (5 μg/ml)
Immunoblotting (2.5 μg/ml, chemiluminescence)
Paraffin Sections (2.5 μg/ml, pressure cooker pre-treatment required)

Stato fisico

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Risultati analitici

Negative Control
Brain tissue
Positive Control
Doxorybicin-treated MCF-7 cells (see comments) or intestinal tissue

Altre note

The immunoblotting application applies to human samples only. Mouse and rat frozen intestinal tissue was fixed with Zamboni′s fixative prior to staining. Only random cells will stain for bcl-x in brain tissue. For MCF-7 cells as a positive control, stimul

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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M González-García et al.
Development (Cambridge, England), 120(10), 3033-3042 (1994-10-01)
Most examples of cell death in animals are controlled by a genetic program that is activated within the dying cell. The apoptotic process is further regulated by a set of genes that act as repressors of cell death. Of these
L H Boise et al.
Cell, 74(4), 597-608 (1993-08-27)
We report the isolation of bcl-x, a bcl-2-related gene that can function as a bcl-2-independent regulator of programmed cell death (apoptosis). Alternative splicing results in two distinct bcl-x mRNAs. The protein product of the larger mRNA, bcl-xL, is similar in
W Fang et al.
Journal of immunology (Baltimore, Md. : 1950), 155(1), 66-75 (1995-07-01)
Developing lymphocytes undergo extensive cell death during selection of the immune repertoire. We investigated the influence of bcl-xL, a member of the bcl-2 family of apoptosis regulatory genes, on apoptosis in a model system for negative selection in the B
S Krajewski et al.
Cancer research, 54(21), 5501-5507 (1994-11-01)
The in vivo patterns of bcl-X gene expression were assessed in human and mouse tissues using an immunohistochemical approach. Polyclonal antisera were raised against synthetic peptides corresponding to amino acids 46-66 and 61-79 of the human Bcl-X protein and were
R Datta et al.
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, 6(4), 363-370 (1995-04-01)
Acquired resistance to diverse chemotherapeutic agents has been associated with overexpression of the P-glycoprotein. We have selected human U-937 cells for clones resistant to the cytotoxic agents doxorubicin (U-A20) and vincristine (U-V20). The results demonstrate that P-glycoprotein-positive U-A20 and U-V20

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