MABS1187

Anti-phospho-GADS (Thr262) Antibody, clone 6E11.2

clone 6E11.2, from mouse

• Sinonimo/i: GRB2-related adapter protein 2, Thr262 phosphorylated, Adapter protein GRID, Thr262 phosphorylated, GRAP-2, Thr262 phosphorylated, GRB-2-like protein, Thr262 phosphorylated, Grb2-related adapter protein downstream of Shc, Thr262 phosphorylated, GRB2L, Th
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 6E11.2, monoclonal
• Reattività contro le specie: human
• tecniche: western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_001278753
• N° accesso UniProt: O75791
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pThr262)
• Informazioni sul gene: human ... GRAP2(9402)

Descrizione

Descrizione generale

GRB2-related adapter protein 2 (UniProt O75791; also known as Adapter protein GRID, GRAP-2, GRB-2-like protein, Grb2-related adapter protein downstream of Shc, GRB2L, GRBLG, GRBX, Grf-40, Grf40 adapter protein, Growth factor receptor-binding protein, Hematopoietic cell-associated adapter protein GrpL, P38, Protein GADS, SH3-SH2-SH3 adapter Mona) is encoded by the GRAP2 (also known as GADS, GRB2L, GRID) gene (Gene ID 9402) in human. GADS (Grb2-related adapter protein downstream of Shc), GRB2 and GRAP constitute the GRB2 family of adapter proteins characterized by a central SH2 domain (a.a. 58-149 of GADS) flanked between two SH3 domains (a.a. 1-56, 271-330 of GADS). Although the SH2 domains of all three members have similar binding specificities, the C-terminal SH3 domain of GADS possesses unique binding specificity that allows for its interaction with the adapter protein SLP-76 (SH2 domain-containing leukocyte protein of 76 kDa). The GADS-SLP-76 complex is recruited to tyrosine-phosphorylated LAT (linker for activation of T cells) via GADS SH2 domain, allowing the assembly of signaling complexes critical to T-cell activation. In addition, evidences indicate that GADS plays a critical role in the development of BCR-ABL-mediated lymphoid disease. The oncogenic Bcr-Abl fusion protein is shown to bind GADS in a phosphotyrosine-dependent manner and GADS is expressed in a subset of CML cell lines and B-ALL patient samples, where it associates with both BCR-ABL and SLP-76.

Specificità

Clone 6E11.2 detects GADS phosphorylated on Thr262. The corresponding phosphorylation site in human GADS spliced isoform 2 is Thr149 (UniProt O75791-2).

Immunogeno

Epitope: pThr262.

KLH-conjugated linear peptide corresponding to a human GADS sequence containing phosphorylated Thr262.

Applicazioni

Anti-phospho-GADS (Thr262) Antibody, clone 6E11.2 is an antibody against phospho-GADS for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Immunological Signaling

Qualità

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected GADS Thr262 phosphorylation induction in 10 µg lysate from Calyculin A- and TNFα-treated Jurkat cells.

Descrizione del bersaglio

~40 kDa observed. 37.91 kDa (isoform 1) and 24.38 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable