MABN2436

Anti-Guanylate Cyclase 2E Antibody, clone 8A5

clone 8A5, from mouse

• Sinonimo/i: RETGC-1, Guanylate cyclase-E, Guanylate cyclase-2E, GC-E
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 8A5, monoclonal
• Reattività contro le specie: bovine, mouse, human
• Confezionamento: antibody small pack of 25 μg
• tecniche: immunofluorescence: suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_032218
• N° accesso UniProt: P52785
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Gucy2E(14919)

Descrizione

Descrizione generale

Guanylyl cyclase GC-E (UniProt: P52785; also known as EC: 4.6.1.2, Guanylate cyclase 2E, GC-2E) is encoded by the Gucy2e (also known as Guc2e) gene (Gene ID: 14919) in murine species. GC-2E is a single-pass type I membrane protein that plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the re-synthesis of cGMP required for recovery of the dark state after phototransduction. Following photoexcitation, cGMP in photoreceptors is first hydrolyzed by a light-activated phosphodiesterase (PDE6) and then replenished by a retinal membrane guanylyl cyclase. It is synthesized with a 50 amino acid signal peptide that is cleaved to generate the mature enzyme. It consists of an extracellular domain (aa 51-469), a helical domain (aa 470-490) and a cytoplasmic domain (aa 491-1108).

Specificità

Clone 8A5 detects Guanylate cyclase-1 in Bovine, Human, and murine species. It targets an epitope within 48 amino acids from the C-terminal end.

Immunogeno

Epitope: C-terminus

GST-tagged recombinant fragment corresponding to 48 amino acids from the C-terminal end of human Guanylate cyclase-1.

Applicazioni

Anti-Guanylate Cyclase-2E, clone 8A5, Cat. No. MABN2436, is a highly specific mouse monoclonal antibody that targets retinal Guanylyl cyclase (RETGC-1; GC-2E) and has been used in Immunofluorescence and Western Blotting.

Immunofluorescence Analysis: A representative lot detected Guanylate Cyclase-1 in mouse retina tissues (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).

Immunofluorescence Analysis: A representative lot detected Guanylate Cyclase-1 in Immunofluorescence applications (Molday, L.L., et. al. (2013). Hum Mol Genet. 22(19):3894-905).

Western Blotting Analysis: 10 ug/mL of a representative lot detected Guanylate Cyclase-1 in mouse retina tissue lysates (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).

Western Blotting Analysis: A representative lot detected Guanylate Cyclase-1 in Western Blotting applications (Peshenko, I.V., et. al. (2011). Biochemistry. 50(44):9511-9; Molday, L.L., et. al. (2013). Hum Mol Genet. 22(19):3894-905)

Research Category
Neuroscience

Qualità

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .

Descrizione del bersaglio

~120 kDa observed; 120.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable