MABN1818

Anti-α-Synuclein (SNCA) Antibody

mouse monoclonal, SOY1

• Sinonimo/i: Alpha-synuclein, NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Synuclein alpha-140
• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Nome del prodotto: Anti-α-Synuclein Antibody, clone SOY1, clone SOY1, from mouse
• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: SOY1, monoclonal
• Reattività contro le specie: mouse, human
• Reattività contro le specie (prevista in base all’omologia): rat (based on 100% sequence homology)
• tecniche: ELISA: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_000336
• N° accesso UniProt: P37840
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SNCA(6622)

Descrizione

Descrizione generale

Alpha-synuclein (UniProt P37840; also known as NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Synuclein alpha-140) is encoded by the SNCA (also known as NACP, PARK1, PARK4) gene (Gene ID 6622) in human. Pathological aggregates are common features of many neurodegenerative diseases, such as tau neurofibrillary tangles (NFTs) in Alzheimer’s disease (AD) and frontotemporal degeneration, and α-synuclein (α-syn or αS) Lewy bodies (LBs) in Parkinson’s disease (PD) and dementia with LBs (DLB). Alpha-synuclein is a phospholipid-binding protein concentrated in presynaptic terminals where it promotes SNARE complex formation and modulates synaptic functions. Alpha-synuclein is the major component of pathologic inclusions that characterize PD, DLB, and multiple system atrophy (MSA). Research shows that αS exists not only as unfolded monomers, but in large part also as multimers, principally as ~60 kDa tetramers composed of four N-acetylated αS, that assume α-helical conformation and resist aggregation. PD-causing αS missense mutations are found to shift cellular αS from tetramers/multimers to monomers, indicating that decreased α-helical tetramers and increased unfolded monomers initiate pathogenesis. In addition, both casein kinase-1 (CK-1) and CK-2 can catalyze the phosphorylation of αS on Ser129, and Ser129-phosphorylated αS is found in αS inclusions.

Specificità

Clone SOY1 detected both wild-type alpha-synuclein and fPD mutants (Dettmer, U., et al. (2015). Nat. Commun. 6:7314). Clone SOY1 targets a C-terminal half epitope present in all three human spliced iisoforms, NACP140, NACP112, and NACP126, reported by UniProt (P37840). Clone SOY1 also exhibits murine cross-reactivity, albeit at a lower sensitivity than that of human alpha-synuclein.

Immunogeno

Purified human erythrocyte alpha-synuclein.

Applicazioni

Anti-α-Synuclein, clone SOY1 Antibody, Cat. No. MABN1818, is a highly specific mouse monoclonal antibody that targets -synuclein and has been tested in ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected α-synuclein in human cerebellum, cerebral cortex, and pancreas tissue sections.

ELISA Analysis: A 1:74.6 dilution from a representative lot (preconjugated with Sulfo tag) detected recombinant human α-synuclein (0.2-40 ng/mL) captured by clone 2F12 (Cat. No. MABN1817; 200 ng/30 µL/well for coating) in a sandwich ELISA application (Courtesy of Tim Bartels, Ph.D., Brigham and Women′s Hospital, Boston, MA, U.S.A.).

Immunoprecipitation Analysis: 4 µL from a representative lot immunoprecipitated α-synuclein from 50 µg of HEL human erythroleukemia cell lysate (Courtesy of Tim Bartels, Ph.D., Brigham and Women′s Hospital, Boston, MA, U.S.A.).

ELISA Analysis: A representative lot (preconjugated with Sulfo tag) detected both endogenous alpha-synuclein (αS) from human cortical homogenate, as well the exogenously expressed wild type and familial PD (fPD) αS mutants (A30P, E46K, H50Q, G51D, A53T) from sytosolic extracts of transfected M17D human neuroblastoma cells in a sandwich ELISA application utilizing clone 2F12 (Cat. No. MABN1817) as the capture antibody (Dettmer, U., et al. (2015). Nat. Commun. 6:7314).

ELISA Analysis: A representative lot (preconjugated with Sulfo tag) detected both pre-aggregated fibrillar recombinant -synuclein as well as partially purified Lewy bodies (LBs) from a DLB (dementia with LBs) patient with or without prior sample denaturing by boiling with 2% SDS in a sandwich ELISA application utilizing clone 2F12 (Cat. No. MABN1817) as the capture antibody (Dettmer, U., et al. (2015). Nat. Commun. 6:7314).

Qualità

Evaluated by Western Blotting in human fetal brain tissue lysate.

Western Blotting Analysis: A 1:125 dilution of this antibody detected α-synuclein in 10 µg of human fetal brain tissue lysate.

Descrizione del bersaglio

~14.5 kDa observed. 14.46/11.37/13.11 kDa (human isoform NACP140/NACP112/NACP126) calculated.. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable