MABF951

Anti-IL-2R beta (CD122) Antibody, clone TM-beta1

clone TM-beta 1, from rat

• Sinonimo/i: Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL-2R subunit beta, High affinity IL-2 receptor subunit beta, p70-75, CD122
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rat
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: TM-beta 1, monoclonal
• Reattività contro le specie: mouse
• Confezionamento: antibody small pack of 25 μL
• tecniche: flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; neutralization: suitable; western blot: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_032394.1
• N° accesso UniProt: P16297
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Il2Rb(16185)

Descrizione

Descrizione generale

Interleukin-2 receptor subunit beta (UniProt: P16297; also known as IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, High affinity IL-2 receptor subunit beta, p70-75, CD122) is encoded by the Il2rb gene (Gene ID: 16185) in murine species. IL-2R is a non-covalent dimer of an alpha and a beta subunit. It can exist as a high affinity dimer, an intermediate affinity monomer of beta subunit, and as a low affinity monomer of alpha subunit. IL-2R beta is a single-pass type I membrane protein. The beta subunit is involved in receptor mediated endocytosis and it transduces the mitogenic signals of IL-2. IL-2R beta is synthesized with a signal peptide (aa 1-26), which is cleaved off to produce the mature form that has an extracellular domain (aa 27-240), a transmembrane domain (aa 241-268), and a long cytoplasmic domain (aa 269-539). IL-2R beta contains a WSXWS motif (aa 221-225), which is essential for its proper folding and thereby efficient intracellular transport. It also has a Box 1 motif (aa 281-289) that is required for JAK interaction and/or activation.

Specificità

Clone TM-beta 1 is a rat monoclonal antibody that detects IL-2R beta subunit in murine species.

Immunogeno

TART-mB whole cells

Applicazioni

Anti-IL-2R beta (CD122), clone TM-beta1, Cat. No. MABF951, is a rat monoclonal antibody that detects IL-2R beta and has been tested for use in Flow Cytometry, Function assay, Immunocytochemistry, Immunoprecipitation, Neutralizing, and Western Blotting.

Neutralizing Analysis: Freshly isolated mouse splenocytes pretreated with different amounts of MABF951, Anti-IL-2R beta (CD122), clone TM- 1 showed a co-relation of the amount of antibody used vs percent of cells stained with FITC-labeled antibody. In antibody titration, as the concentration of unlabeled anti-IL-2R beta (CD122) is decreased less inhibition is observed as a result.

Inhibits Activity/Function: A representative lot abolished the binding of IL2 ligand to IL-2R beta (CD122). (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8; Yokoyama, S., et. al. (2009). Proc Natl Acad Sci USA. 106(37):15849-54).

Flow Cytometry Analysis: A representative lot detected IL-2R beta (CD122) in Flow Cytometry applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).

Western Blotting Analysis: A representative lot detected IL-2R beta (CD122) in Western Blotting applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).

Immunoprecipitation Analysis: A representative lot immunoprecipitated IL-2R beta (CD122) in Immunoprecipitation applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).

Immunocytochemistry Analysis: A representative lot detected IL-2R beta (CD122) in Immunocytochemistry applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).

Research Category
Inflammation & Immunology

Qualità

Isotype testing: Identity Confirmation by Isotyping test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b.

Descrizione del bersaglio

60.54 kDa calculated

Stato fisico

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2b in PBS without azide.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.