MABE1115M

Anti-Pax1 Antibody, clone 5A2

clone 5A2, from rat

• Sinonimo/i: Paired box protein Pax-1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rat
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 5A2, monoclonal
• Reattività contro le specie: human, mouse
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_032806
• N° accesso UniProt: P09084
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Pax1(18503)

Descrizione

Descrizione generale

Paired box protein Pax-1 (UniProt: P09084) is encoded by the Pax1 (also known as Pax-1) gene (Gene ID: 18503) in murine species. Pax-1 is a transcriptional activator that plays a role in the formation of segmented structures of the embryo and has important role in the normal development of the vertebral column. Mutations in Pax1 gene are associated with vertebral malformations. Undulated homozygous mice exhibits vertebral malformations along the entire rostro-caudal axis. Pax genes are vital for the specification of progenitor cells and for maintenance of progenitor cell fate, including that of cancer cells. Pax1 gene is silenced by methylation in ovarian and cervical cancers and may serve as a tumor suppressor gene. The methylation levels of Pax1 gene is significantly higher in tumor tissues compared to non-tumor paracancerous tissues. Hence, methylation status of Pax1 may serve as useful biomarkers for the detection of cervical cancer and is also a good prognostic indicator for oral squamous cell carcinoma. (Ref.: Huang J et al (2017). Int. J. Environ. Res. Public Health. 14(2). pii: E216).

Specificità

Clone 5A2 detects Paired box protein Pax-1 in murine tissues.

Immunogeno

GST-tagged full-length murine recombinant paired box protein Pax-1.

Applicazioni

Anti-Pax1, clone 5A2, Cat. No. MABE1115, is a highly specific rat monoclonal antibody that targets Paired box protein Pax-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Western Blotting Analysis: A representative lot detected Pax1 in Western Blotting applications (Feederle, R., et. al. (2016). Monoclon Antib Immunodiagn Immunother. [Epub ahead of print]).

Immunohistochemistry Analysis: A representative lot detected Pax1 in Immunohistochemistry applications (Feederle, R., et. al. (2016). Monoclon Antib Immunodiagn Immunother. [Epub ahead of print]; Kist, R., et. al. (2014). PLoS Genet. 10(10):e1004709).

Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected Pax1 in Embryonic mouse head (E14.5) (Courtesy of Dr. Heiko Peters, Ph.D., Institute of Genetic Medicine, Newcastle University, United Kingdom).

Western Blotting Analysis: A 1:10 dilution from a representative lot detected Pax1 in d11.5 embryonic vertebral column tissue lysates (Courtesy of Dr. Heiko Peters, Ph.D., Institute of Genetic Medicine, Newcastle University, United Kingdom).

Qualità

Evaluated by Western Blotting in mouse esophagus tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Pax1 in 10 µg of mouse esophagus tissue lysate.

Descrizione del bersaglio

~46 kDa observed; 46.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable