MABC43
Anti-Mcl-1 Antibody, clone RC13
clone RC13, from mouse
Sinonimo/i:
myeloid cell leukemia sequence 1 (BCL2-related), myeloid cell leukemia sequence 1, isoform 1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, myeloid cell leukemia ES, induced myeloid leukemia cell differentiation protein Mcl-1
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
RC13, monoclonal
Reattività contro le specie
human
Confezionamento
antibody small pack of 25 μL
tecniche
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG1κ
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
ambient
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... MCL1(4170)
Descrizione generale
Mcl-1 (Induced myeloid leukemia cell differentiation protein) is a potential transmembrane protein associated with the mitochondria belonging to the Bcl-2 family of proteins. Members of the Bcl-2 family have known participation in regulatory roles for apoptosis, cell growth, and differentiation. The hydrophobic carboxyl terminus is conserved among members of this family and in other members has proven to be a transmembrane region. Mcl-1 is found to be expressed early in myeloid differentiation. Two separate isoforms of Mcl-1 exist with opposing functions. Full length Mcl-1 (Isoform 1) promotes cell viability, over proliferation. Isoform 2 results from the skipping of the central exon of Mcl-1 during mRNA processing and the joining of the first and third exons. The function of the resulting protein induces apoptosis when accumulated. Pro-apoptotic function is shown to increase when isoform 1 and 2 dimerize.
Immunogeno
Recombinant protein corresponding to human Mcl-1.
Applicazioni
Detect Mcl-1 using this Anti-Mcl-1 Antibody, clone RC13 validated for use in WB, IH & IP.
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in follicular lymphoma, diffuse large cell lymphoma, and polymorphic lymphoma tissues. (Zhou, P., et al. (2001). Blood. 97(12):3902-3909).
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP (Han, J., et al. (2005). Biological Chemistry. 280:16383-16392).
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP (Han, J., et al. (2005). Biological Chemistry. 280:16383-16392).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
BCL2 & Inhibition
Qualità
Evaluated by Western Blot in Raji cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Mcl-1 in 10 µg of Raji cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Mcl-1 in 10 µg of Raji cell lysate.
Descrizione del bersaglio
~37 kDa observed
Linkage
Replaces: MAB4602
Stato fisico
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Risultati analitici
Control
Raji cell lysate
Raji cell lysate
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Qi Shen et al.
Cell death & disease, 9(10), 986-986 (2018-09-27)
Lung cancer is the leading cause of death in the United States, with non-small cell lung cancers (NSCLC) accounting for 85% of all cases. By analyzing the expression profile of the pro-apoptotic and anti-apoptotic proteins, we have assigned NSCLCs into
Raju R Rayavarapu et al.
The Journal of biological chemistry, 290(14), 8722-8733 (2015-02-15)
The metastasis of cancer cells from the site of the primary tumor to distant sites in the body represents the most deadly manifestation of cancer. In order for metastasis to occur, cancer cells need to evade anoikis, which is defined
Adriana E Tron et al.
Nature communications, 9(1), 5341-5341 (2018-12-19)
Mcl-1 is a member of the Bcl-2 family of proteins that promotes cell survival by preventing induction of apoptosis in many cancers. High expression of Mcl-1 causes tumorigenesis and resistance to anticancer therapies highlighting the potential of Mcl-1 inhibitors as
Lindy Vernooij et al.
Molecular cancer therapeutics, 20(6), 1161-1172 (2021-04-15)
Neuroblastoma tumors frequently overexpress the anti-apoptotic protein B-cell lymphoma/leukemia 2 (BCL-2). We previously showed that treating BCL-2-dependent neuroblastoma cells with the BCL-2 inhibitor venetoclax results in apoptosis, but unfortunately partial therapy resistance is observed. The current study describes the identification
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