MABC1227

Anti-PIP4K2B (PIP5K2B) Antibody, clone 7D10.1

clone 7D10.1, from mouse

• Sinonimo/i: Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, 1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PI(5)P 4-kinase type II beta, PIP4KII-beta, PtdIns(5)P-4-
• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Origine biologica: mouse
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 7D10.1, monoclonal
• Reattività contro le specie: mouse
• Reattività contro le specie (prevista in base all’omologia): human (based on 100% sequence homology)
• tecniche: western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_003550
• N° accesso UniProt: P78356
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... PIP4K2B(8396)

Descrizione

Descrizione generale

Phosphatidylinositol 5-phosphate 4-kinase type-2 beta (EC 2.7.1.149; UniProt P78356; also known as 1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PI(5)P 4-kinase type II beta, PIP4KII-beta, PIP4K2B, PtdIns(5)P-4-kinase isoform 2-beta) is encoded by the PIP4K2B (also known as PIP5K2B, PIP5KIIB) gene (Gene ID 8396) in human. PIP4K2B catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 5-phosphate to 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate. It is highly expressed in brain, heart, pancreas, skeletal muscle and kidney, however, lower levels are detected in placenta, lung, and liver. Mice deficient in PIP4K2B are shown to be hypersensitive to insulin and exhibit reduced body weights compared to wild-types. Adult male mice lacking PIP4K2B have significantly less body fat, but female mice show increased sensitivity to insulin in the presence of normal adiposity. Mice lacking PIP4K2B exhibit enhanced in vivo insulin-induced activation of Akt in the skeletal muscle and liver. Ref.: Lamia, KA., et al. (2004). Mol. Cell 24, 5080-5087

Specificità

Epitope region is present in spliced isoform 1, but not in isoform 2 reported by UniProt (P78356).

Immunogeno

GST-tagged recombinant fragment corresponding to a region within the C-terminal half of human PIP4K2B (PIP5K2B).

Applicazioni

Detect PIP4K2B using this mouse monoclonal Anti-PIP4K2B (PIP5K2B) Antibody, clone 7D10.1, Cat. No. MABC1227, validated for use in Western Blotting.

Research Category
Apoptosis & Cancer

Qualità

Evaluated by Western Blotting in L-929 mouse fibroblast lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PIP4K2B (PIP5K2B) in 10 µg of L-929 mouse fibroblast lysate.

Descrizione del bersaglio

~48 kDa observed. 47.25/47.19 kDa (human/mouse) calculated (Met 1 removed). Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Protein G purified.

Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable