MABC1211

Anti-Sorting Nexin-17 Antibody, clone 4B8.1

clone 4B8.1, from mouse

• Sinonimo/i: Sorting nexin-17, SNX17
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 4B8.1, monoclonal
• Reattività contro le specie: human
• tecniche: immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_001253989
• N° accesso UniProt: Q15036
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SNX17(9784)

Descrizione

Descrizione generale

Sorting nexin-17 (UniProt Q15036) is encoded by the SNX17 (also known as KIAA0064) gene (Gene ID 9784) in human. The sorting nexins (SNXs) constitute a family of adaptor proteins involved in proteins trafficking through the endosomal pathway. Originally identified as a binding partner of the cell adhesion protein P-selectin, sorting nexin-17 (SNX17) has since been shown to mediate internalization and recycling of multiple cell surface proteins including P-selectin, amyloid precursor protein (APP), integrin β1, low density lipoprotein receptor (LDLR), LDLR related protein (Lrp1), ApoER2, and FEEL1. All SNX proteins contain a conserved phox homology (PX) domain that mediates phosphoinositides binding in a SNX type-specific manner, as well as various scaffolding modules including SH3, BAR, and PDZ domains. SNX17 PX domain (a.a. 1-109) displays specificity for phosphatidylinositol 3-monophosphate, which is enriched at the cytoplasmic face of the early endosome. SNX17 also contains a 4.1 protein/ezrin/radixin/moesin (FERM) domain (a.a. 115-432) comprised of an ubiquitin/Ras association (RA) subdomain known as the F1 lobe (a.a. 113-205), an F2 lobe (a.a. 206-269) with a helical structure similar to acyl-CoA binding protein, and an F3 lobe (a.a. 270-388) similar to the phosphotyrosine binding (PTB)/pleckstrin homology domain. The FERM domain mediates interaction with Asn-Pro-Xaa-Tyr/Phe (NPX(Y/F) motif-containing proteins for internalization as well as interaction with adaptor proteins-containing PTB (or FERM) domains to trigger signaling events.

Specificità

Clone 4B8.1 recognizes an internal epitope within the FERM-like region present in both spliced isoforms of human sorting nexin-17 reported by UniProt (Q15036).

Immunogeno

Epitope: Within the FERM-like region.

GST-tagged recombinant fragment from the FERM-like region of human Sorting Nexin-17.

Applicazioni

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Sorting Nexin-17 in human pancreas, prostate, and cerebral cortex tissue sections.

Research Category
Apoptosis & Cancer

Research Sub Category
Vesicular Trafficking

This Anti-Sorting Nexin-17 Antibody, clone 4B8.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of Sorting Nexin-17.

Qualità

Evaluated by Western Blotting in U251 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Sorting Nexin-17 in 10 µg of U251 cell lysate.

Descrizione del bersaglio

~52 kDa observed. 52.90 kDa (isoform 1) and 50.00 kDa (isoform 2) calculated.

Stato fisico

Format: Purified

Protein G purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable