MABC1207

Anti-CIRP (CIRBP) Antibody, clone 6C7.1

clone 6C7.1, from mouse

• Sinonimo/i: Cold-inducible RNA-binding protein, A18 hnRNP, Glycine-rich RNA-binding protein CIRP
• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 6C7.1, monoclonal
• Reattività contro le specie: rat, human
• Reattività contro le specie (prevista in base all’omologia): mouse (based on 100% sequence homology)
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_001271
• N° accesso UniProt: Q14011
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CIRBP(1153)

Descrizione

Descrizione generale

Cold-inducible RNA-binding protein (UniProt Q14011; also known as A18 hnRNP, Glycine-rich RNA-binding protein CIRP) is encoded by the CIRBP (also known as A18HNRNP, CIRP) gene (Gene ID 1153) in human. CIRP is an 18-kDa RNA-binding protein with an RNA recognition motif (RRM; a.a. 6-84) and a glycine-rich domain (a.a. 92-158). CIRP migrates from nucleus to cytoplasm, where it mediates posttranscriptional regulation of target mRNAs. Originally characterized and named as a cold-induced protein, CIRP exerts antiapoptotic and cell-protective effects in response to cold stress, as well as other stimuli such as UV irradiation and hypoxia. CIRP is expressed in a broad range of tissues (e.g. testis, brain, lung, kidney, liver, stomach, bone marrow, and heart) and upregulated CIRP levels have been reported in several types of cancers. CIRP knockdown is reported to increase p53 level and enhance cancer cells chemo-sensitivity to DNA damage inducing drugs. Likewise, CIRP overexpression results in p53 downregulation.

Specificità

Clone 6C7.1 is expected to recognize CIRP spliced isoforms 1 and 3 (UniProt Q14011). Cross-reactivity toward spliced isoform 2 is possible, but has not been confirmed.

Immunogeno

GST-tagged recombinant human CIRP (CIRBP) internal fragment.

Applicazioni

Detect CIRBP using this mouse monoclonal Anti-CIRP (CIRBP) Antibody, clone 6C7.1, Cat. No. MABC1207, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected CIRP (CIRBP) in rat testis and human prostate cancer, pancreas, tonsil tissue sections.

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected CIRP (CIRBP) in 10 µg of Raji human B-cell lymphoma cell lysate.

Research Category
Apoptosis & Cancer

Qualità

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected CIRP (CIRBP) in 10 µg of SH-SY5Y human neuroblastoma cell lysate.

Descrizione del bersaglio

~18 kDa observed. 18.65/28.36/26.13 kDa (isoform 1/2/3) calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Protein G purified.

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable