MAB88079
Anti-Heat Shock Factor II Antibody, clone 3E2
clone 3E2, Chemicon®, from rat
Sinonimo/i:
HSFII
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Origine biologica
rat
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
3E2, monoclonal
Reattività contro le specie
human, monkey, mouse
Produttore/marchio commerciale
Chemicon®
tecniche
ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG2a
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... HSF2(3298)
Specificità
MAB88079 recognizes a protein of 70 kDa, identified as Heat Shock Factor 2 (HSF2). The synthesis of heat shock proteins is dependent on transcriptional and post-transcriptional control in eukaryotic cells. The regulation of heat shock gene transcription is mediated by the transcriptional activator, Heat Shock Factor (HSF), which binds to heat shock response elements (HSEs). At least three HSFs have been isolated from the human, mouse, chicken, and tomato genomes and an additional factor, HSF4 has been identified in humans. HSFs purified from yeast, Drosphila, and human have different molecular weights and do not cross-react immunologically. These factors are activated by distinct stimuli. HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents whereas, the HSF2 is activated during differentiation. some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA. the phosphorylation of HSF1 may be necessary for maximal transcription of heat shock genes.
Immunogeno
Full length recombinant mouse HSF2 proteins (aa 1-517)
Applicazioni
Anti-Heat Shock Factor II Antibody, clone 3E2 is an antibody against Heat Shock Factor II for use in ELISA, IF, IP & WB.
Immunofluorescence Immunohistology (predominantly cytoplasmic with some nuclear). Immunoprecipitation: 2 μg/mg protein lysate. Western blot. 1:200-1:400 ELISA
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Stato fisico
Format: Purified
Purified immunoglobulin. Liquid containing 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.
Stoccaggio e stabilità
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Risultati analitici
Control
POSITIVE CONTROL:
NIH-3T3 cells
POSITIVE CONTROL:
NIH-3T3 cells
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Nitzan Levy et al.
Endocrinology, 148(7), 3449-3458 (2007-03-31)
Estrogen receptors (ERs) regulate transcription by interacting with regulatory elements in target genes. However, known ER regulatory elements cannot explain the expression profiles of genes activated by estradiol (E2) and selective estrogen receptor modulators (SERMs). We previously showed that the
Linda Pesonen et al.
Cell stress & chaperones, 26(5), 819-833 (2021-08-01)
Cancer cells rely on heat shock proteins (HSPs) for growth and survival. Especially HSP90 has multiple client proteins and plays a critical role in malignant transformation, and therefore different types of HSP90 inhibitors are being developed. The bioactive natural compound
Marek A Budzyński et al.
Molecular and cellular biology, 35(14), 2530-2540 (2015-05-13)
In mammals the stress-inducible expression of genes encoding heat shock proteins is under the control of the heat shock transcription factor 1 (HSF1). Activation of HSF1 is a multistep process, involving trimerization, acquisition of DNA-binding and transcriptional activities, which coincide
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