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MAB5740

Sigma-Aldrich

Anti-Radial Glial Cell Marker-2 Antibody, clone RC2

clone RC2, from mouse

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

RC2, monoclonal

Reattività contro le specie

mouse

tecniche

immunohistochemistry: suitable

Isotipo

IgMλ

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

The monoclonal antibody Radial Glial Cell Marker-2, clone RC2 (RC2) was generated in mouse by conventional hybridoma methodology. The antigen recognized by RC2 is robust, allowing aldehyde fixation appropriate to high resolution light and electron microscopic analyses. From the neural tube stage of fetal development the antibody delineates throughout the central nervous system a subpopulation of neuroepithelial cells which have a radial bipolar morphology. RC2 also recognizes monopolar cell forms in the spinal cord and the cerebellum as early as E15, and in the dentate gyrus of the hippocampal formation from the day of birth. Monopolar forms in the cerebellum are inferred to be progenitors of Bergmann glia. The robustness of the antigen recognized by RC2 makes this probe a valuable tool to study the morphological transformations of the bipolar radial glia during their mitotic turnover. It also provides a sensitive stain for the study of the organization and the histogenetic role of the overall radial fiber system (3).

Specificità

Detects radial glial cell marker-2
Other species have not been tested.

Immunogeno

Embryonic rat brain

Applicazioni

Anti-Radial Glial Cell Marker-2 Antibody, clone RC2 detects level of Radial Glial Cell Marker-2 & has been published & validated for use in IH.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Stato fisico

Ammonium sulfate purified mouse monoclonal in PBS with 0.05% NaN3.
Format: Purified

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Hitomi Aoki et al.
Development (Cambridge, England), 139(4), 667-677 (2012-01-14)
Rest (RE1-silencing transcription factor, also called Nrsf) is involved in the maintenance of the undifferentiated state of neuronal stem/progenitor cells in vitro by preventing precocious expression of neuronal genes. However, the function of Rest during neurogenesis in vivo remains to
Expression and immunolocalization of Gpnmb, a glioma-associated glycoprotein, in normal and inflamed central nervous systems of adult rats.
Huang, JJ; Ma, WJ; Yokoyama, S
Brain and Behavior null
Lei Wang et al.
Nature neuroscience, 19(7), 888-896 (2016-05-24)
The unique mental abilities of humans are rooted in the immensely expanded and folded neocortex, which reflects the expansion of neural progenitors, especially basal progenitors including basal radial glia (bRGs) and intermediate progenitor cells (IPCs). We found that constitutively active
Jan Mulder et al.
The European journal of neuroscience, 31(12), 2166-2177 (2010-06-10)
The Ca(2+)-binding proteins (CBPs) calbindin D28k, calretinin and parvalbumin are phenotypic markers of functionally diverse subclasses of neurons in the adult brain. The developmental dynamics of CBP expression are precisely timed: calbindin and calretinin are present in prospective cortical interneurons
Suyan Li et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(37), 14809-14815 (2013-09-13)
Current models of brain development support the view that VEGF, a signaling protein secreted by neuronal cells, regulates angiogenesis and neuronal development. Here we demonstrate an autonomous and pivotal role for endothelial cell-derived VEGF that has far-reaching consequences for mouse

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