MAB4100
Anti-MRP1 Antibody, clone QCRL-1
clone QCRL-1, Chemicon®, from mouse
Sinonimo/i:
Multidrug Resistance Protein-1
Autenticati per visualizzare i prezzi organizzativi e contrattuali.
Scegli un formato
Cambia visualizzazione
Informazioni su questo articolo
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
QCRL-1, monoclonal
Species reactivity:
human
Application:
FACS, ICC, IHC (p), WB
Citations:
19
Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutartibiological source
mouse
Quality Segment
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
QCRL-1, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Gene Information
human ... ABCC1(4363)
Immunogen
Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1 (Cole et al., 1992; Hipfner et al., 1994, 1999).
Application
Anti-MRP1 Antibody, clone QCRL-1 detects level of MRP1 & has been published & validated for use in FC, WB, IC, IH, IH(P).
Western Blot: 0.2 μg/mL, with HRP-conjugated anti mouse IgG (Hipfner et al., 1994, 1996).
Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).
Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).
Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).
Immunoprecipitation (Hipfner, et al., 1994; 1996).
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).
Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).
Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).
Immunoprecipitation (Hipfner, et al., 1994; 1996).
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Reacts with a defined linear epitope (aa 918-924) of MRP1, a 190 kDa transmembrane
phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines
and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,
MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.
phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines
and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,
MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.
Physical form
Format: Purified
Purified immunoglobulin. Liquid in PBS with 0.1% sodium azide.
Preparation Note
Store at 2-8ºC for up to 3 months. For longer term storage, aliquot and freeze at -20ºC.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Still not finding the right product?
Provate il nostro Motore di ricerca dei prodotti per restringere le opzioni.
Classe di stoccaggio
12 - Non Combustible Liquids
wgk
WGK 2
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.