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IP02

Protein A Agarose Suspension

Name: Protein A Agarose Suspension
Brand: MM

Protein A Agarose Suspension designed for immunoprecipitation applications

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Taglio della confezione	SKU	Disponibilità	Prezzo

Informazioni su questo articolo

NACRES:

NA.56

UNSPSC Code:

41116133

Prezzi e disponibilità al momento non sono disponibili

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form

slurry (Liquid)

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze

shipped in

wet ice

storage temp.

2-8°C

Categorie correlate

Antibody Protein Purification

Protein Purification Chromatography

Protein Sample Prep

Protein Biology

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Questo articolo	IP05	IP04	P1925
Millipore IP02 Protein A Agarose Suspension	Millipore IP05 Protein G Plus/Protein A Agarose Suspension	Millipore IP04 Protein G Plus-Agarose Suspension	Millipore P1925 Protein A–Agarose macrobeads
form slurry (Liquid)	form slurry (Liquid)	form slurry (Liquid)	form aqueous suspension
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
contains ≤0.1% sodium azide as preservative	contains ≤0.1% sodium azide as preservative	contains ≤0.1% sodium azide as preservative	contains -
manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename -
storage condition do not freeze	storage condition do not freeze	storage condition do not freeze	storage condition -
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in -

General description

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for mouse IgG_2a and IgG_2b and rabbit IgG.

Protein A conjugated to agarose.

Physical form

In PBS.

Other Notes

Agarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG_2a or IgG_2b monoclonal antibodies. Mouse IgG₁ or IgG₃ and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Contenuto correlato

Agarose Beads for Immunoprecipitation and Antibody Purification

We manufacture IP and antibody purification agarose beads that purify immunoglobulins and IgG fractions. Our kits also provide a total antibody purification process

Numero articolo commerciale globale

SKU	GTIN
IP02-1.5ML	04055977220209

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