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EZHL

Millipore

Human Leptin ELISA Kit

measures and quantify Leptin levels in 25 μL serum or plasma

Sinonimo/i:

Leptin, Obesity factor

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About This Item

Codice UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.32

product name

Human Leptin "Dual Range" ELISA, This Human Leptin "Dual Range" ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.

Livello qualitativo

200

Reattività contro le specie

human

Confezionamento

kit of 1 × 96 wells

Parametri

25 μL sample volume (4hr assay)

assay range

accuracy: 94-114%
intra-assay cv: 1.4-4.9
(Sensitive Assay)
intra-assay cv: 2.6-4.6
linearity: 80-105%
linearity: 96-125%
(sensitive assay)
sensitivity: 0.125 ng/mL
(sensitive assay)
sensitivity: 0.125-20 ng/mL
sensitivity: 0.5 ng/mL
standard curve range: 0.5-100 ng/mL

tecniche

ELISA: suitable

input

sample type cell culture supernatant
sample type plasma (K2 EDTA)
sample type serum

N° accesso NCBI

NM_000230.1

N° accesso UniProt

P41159

applicazioni

research use

Metodo di rivelazione

colorimetric (450nm/590nm)

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Informazioni sul gene

human ... LEP(3952)

Categorie correlate

Descrizione generale

Leptin is a hormone that is secreted by the adipose tissue. It is also produced by the gastrointestinal tract and placenta. Leptin consists of a four-helix bundle. Leptin participates in regulating appetite, body weight, and energy expenditure. Excessive leptin is associated with cardiovascular complications like hypertension, coronary heart disease, and stroke. The LEP gene is mapped on the human chromosome at 7q32.1. Human Leptin "Dual Range" enzyme-linked immunosorbent assay (ELISA) kit is used for the non-radioactive quantification of human leptin in serum, plasma, and other biological media.

Applicazioni

Human Leptin "Dual Range" ELISA kit has been used in the analysis of leptin in:
  • blood samples
  • serum samples
  • plasma samples

Pittogrammi

CorrosionExclamation mark
GHS05,GHS07

Avvertenze

Warning

Indicazioni di pericolo

H290,H317

Classi di pericolo

Met. Corr. 1 - Skin Sens. 1

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

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Seungah Lee et al.
Talanta, 78(2), 608-612 (2009-02-11)
We report a method for the quantitative analysis of human serum leptin, which is a protein hormone associated with obesity, using a nanoarray protein chip based on a single-molecule sandwich immunoassay. The nanoarray patterning of a biotin-probe with a spot
A high-legume low-glycemic index diet reduces fasting plasma leptin in middle-aged insulin-resistant and -sensitive men.
Zhang Z, Lanza E, Ross AC, Albert PS, Colburn NH, Rovine MJ, Bagshaw D, Ulbrecht JS, Hartman TJ
European Journal of Clinical Nutrition null
Preoperative serum leptin levels in patients with breast cancer.
Mehmet Aliustaoglu,Ahmet Bilici,Mahmut Gumus,Aslihan Topal Colak,Gulcan Baloglu et al.
Medical Oncology (Northwood, London, England) null
Gender dimorphism in skeletal muscle leptin receptors, serum leptin and insulin sensitivity.
Borja Guerra,Teresa Fuentes,Safira Delgado-Guerra,Amelia Guadalupe-Grau,Hugo Olmedillas et al.
Testing null
P Prolo et al.
The international journal of biochemistry & cell biology, 30(12), 1285-1290 (1999-01-30)
Leptin is an adipocyte hormone that signals nutritional status to the central nervous system (CNS) and peripheral organs. Leptin is also synthetized in the placenta and in gastrointestinal tract, although its role in these tissues is not yet clear. Circulating
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