ABT1372

Anti-phospho-Keratin-8 Antibody (Ser23)

from rabbit, purified by affinity chromatography

• Sinonimo/i: Keratin, type II cytoskeletal 8, Ser23-phosphorylated, CK-8, Ser23-phosphorylated, Cytokeratin-8, Ser23-phosphorylated, K8, Ser23-phosphorylated, Keratin-8, Ser23-phosphorylated, Type-II keratin Kb8, Ser23-phosphorylated
• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): rhesus macaque (based on 100% sequence homology)
• tecniche: inhibition assay: suitable (peptide); western blot: suitable
• N° accesso NCBI: NP_002264
• N° accesso UniProt: P05787
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: phosphorylation (pSer23)
• Informazioni sul gene: human ... KRT8(3856)

Descrizione

Descrizione generale

Keratin, type II cytoskeletal 8 (UniProt P05787; also known as CK-8, Cytokeratin-8, K8, Keratin-8, Type-II keratin Kb8) is encoded by the KRT8 (also known as CYK8) gene (Gene ID 3856) in human. Cytokeratin-8 (Keratin-8 or K8) belongs to a family of highly conserved epithelial-specific intermediate filament (IF) proteins that play an important role in the mechanical stability and integrity of epithelial cells and tissues. Each epithelium is characterized by its unique keratin expression pattern. Keratin 8 is a type II intermediate filament protein found in simple epithelia, where it pairs with keratin 18 (K8/KI8) and plays an important role in protecting cells from stress, injury, and apoptosis. The structure and function of K8/18 are regulated by posttranslational modifications, including glycosylation, ubiquitination, and phosphorylation. K8 has been reported to be phosphorylated on Ser8, Ser23, Ser73, and Ser431 by various protein kinases. PKCepsilon is reported to mediate K8 phosphorylation on Ser8 and Ser23, p38 and PKCdelta are responsible for K8 Ser73 phosphorylation, while ERK and JNK are involved in K8 Ser431 phosphorylation.

Specificità

This rabbit polyclonal antibody detected the target phospho band only in lysates from phosphatase inhibitors-treated, but not untreated HeLa cells. Antibody blocking by the immunogen peptide, but not the corresponding nonphosphorylated peptide prior to immunoblotting abolished target band detection. The Ser23 designation is based on human keratin-8 sequence with initiator Met1 removed. The phosphorylation site sequence in mouse/rat (-SSR(pS)YT-) differs from that of human (-SSR(pS)FT-) by a single Phe24-to-Tyr24 switch (F24Y). Cross-reactivity of this polyclonal antibody toward murine and rat species is likely, but has not been confirmed.

Immunogeno

KLH-conjugated linear peptide corresponding to the phosphorylation site sequence containing the phosphorylated target serine.

Applicazioni

Peptide Inhibition Assay: Preincubation of a representative lot with the immunogen phosphopeptide, but not the corresponding nonphosphopeptide, abolished target band detection by Western blotting analysis of 10 µg lysate from phosphatase inhibitors-treated HeLa cells.

Research Category
Cell Structure

This rabbit polyclonal Anti-phospho-Keratin-8 (Ser23), Cat. No. ABT1372 is validated for use in Peptide Inhibition Assay and Western Blotting for the detection of Keratin 8 phosphorylated at serine 23.

Qualità

Evaluated by Western Blotting in HeLa cells lysate.

Western Blotting Analysis: A 1:1,000 dilutiion of this antibody detected keratin-8 Ser23 phosphorylation in 10 µg of lysate from phosphatase inhibitors-treated, but not untreated, HeLa cells.

Descrizione del bersaglio

~54 kDa observed. 53.57/56.48 kDa (isoform 1/2) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable