ABE415

Anti-Transcription factor EB Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Transcripiton factor EB, Class E basic helix-loop-helix protein 35, bHLHe35
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), baboon (based on 100% sequence homology), orangutan (based on 100% sequence homology), gibbon (based on 100% sequence homology)
• tecniche: immunoprecipitation (IP): suitable; western blot: suitable
• N° accesso NCBI: NP_009093
• N° accesso UniProt: P19484
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TFEB(7942)

Descrizione

Descrizione generale

The protein named Transcription factor EB (TFEB) or alternatively in older literature, Class E basic helix-loop-helix protein 35 (bHLHe35) and encoded by the human gene TFEB/BHLH35 is a transcription factor that specifically recognizes and binds E-box sequences (5′-CANNTG-3′) on DNA. Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. TFEB in association with the protein TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB also plays a central role in expression of lysosomal genes. TFEB specifically recognizes and binds the CLEAR-box sequence (5′-GTCACGTGAC-3′) present in the regulatory region of many lysosomal genes and through binding acts as a positive regulator of their expression. Finally, TFEB appears to be a positive regulator for autophagy related gene expression, heavy chain immunoglobulin gene expression, and genes necessary for the proper vascularization of important tissues such as the placenta.

Immunogeno

KLH-conjugated linear peptide corresponding to a region near the C-terminus of human Transcription factor EB.

Applicazioni

Detect TFEB using this rabbit polyclonal antibody, Anti-Transcription factor EB Antibody validated for use in western blotting & IP.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Transcription factor EB in 500 µg of Raji cell lysate. Immunopecipitated Transcription factor EB was then detected in Western Blot.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualità

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Transcription factor EB in 10 µg of Raji cell lysate.

Descrizione del bersaglio

~58 kDa and ~40 kDa observed. Uniprot describes two isoforms at 44 kDa and 53 kDa This protein has been observed at 44-53 kDa due to sumoylation (Miller, A. J., et al. (2005). J Biol Chem. 280(1):146-55.).

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable