ABE1947

Anti-PRDM9

from rabbit

• Sinonimo/i: Histone-lysine N-methyltransferase PRDM9, EC: 2.1.1.43, PR domain zinc finger protein 9, PR domain-containing protein 9
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: mouse, human
• Confezionamento: antibody small pack of 25 μg
• tecniche: western blot: suitable
• Isotipo: IgG
• N° accesso NCBI: NP_064612
• N° accesso UniProt: Q9NQV7
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... PRDM9(56979)

Descrizione

Descrizione generale

Histone-lysine N-methyltransferase PRDM9 (UniProt: Q9NQV7; EC: 2.1.1.43, PR domain zinc finger protein 9, PR domain-containing protein 9, PRDM9) is encoded by PRDM9 (also known as PFM6) gene (Gene ID: 56979) in human. PRDM9 is a zinc-finger protein that catalyzes histone H3 Lysine 4 trimethylation (H3K4me3), but lacks capacity to mono- and dimethylate Lysine 4 of histone H3. H3 Lysine -4 methylation plays a vital role in the transcriptional activation of genes during early meiotic prophase. PRDM9 contains multiple domains, including a Kruppel-associated box (KRAB) domain (aa 23-86), an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain (aa 244-358), and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. PRDM9 is reported to be highly polymorphic, both within and between mammalian species. It is shown that most of the naturally occurring sequence polymorphisms in PRDM9 change the identity of the amino acids contacting DNA and/or the number and arrangement of individual fingers in the DNA-binding zinc-finger domains. This allows PRDM9 variants to target a large number of DNA sequences, thereby expanding the distribution of recombination sites. (Ref.: Baker, CL et al. (2015). PLoS Genet 11(9): e1005512).

Specificità

This rabbit polyclonal antibody detects Histone-lysine N-methyltransferase PRDM9 in human and mouse cells. It targets an epitope within 191 amino acids from the N-terminal half.

Immunogeno

GST-tagged recombinant fragment corresponding to 191 amino acids from the N-terminal half of human Histone-lysine N-methyltransferase PRDM9.

Applicazioni

Anti-PRDM9, Cat. No. ABE1947, is a highly specific rabbit polyclonal antibody that targets Histone-lysine N-methyltransferase PRDM9 in human and murine cells and has been tested for use in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A representaive lot detected PRDM9 in Wild type, but not in KO mouse testis tissue lysate ( Courtesy of Guccione Lab @ A*STAR( Institute of Molecular Cell Biology, IMCB) ).

Western Blotting Analysis: A representaive lot detected PRDM9 in Wild type, but not in KO mouse testis tissue lysate (Courtesy of Guccione Lab @ A*STAR(Institute of Molecular Cell Biology, IMCB)).

Qualità

Evaluated by Western Blotting in mouse testis tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected PRDM9 in 10 µg of mouse testis tissue lysate.

Descrizione del bersaglio

~90 kda observed; 103.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Classe di pericolosità dell'acqua (WGK): WGK 1