ABE1047
Anti-NRF2a/GABPA Antibody
serum, from rabbit
Sinonimo/i:
GA-binding protein alpha chain, GABP subunit alpha, Nuclear respiratory factor 2 subunit alpha, Transcription factor E4TF1-60, NRF2a/GABPA
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prezzi e disponibilità al momento non sono disponibili
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
serum
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Reattività contro le specie
human
tecniche
ChIP: suitable
electrophoretic mobility shift assay: suitable
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... GABPA(2551)
Descrizione generale
GA-binding protein alpha chain (UniProt Q06546; also known as GABP subunit alpha, GABPalpha, NRF-2, Nuclear respiratory factor 2 subunit alpha, Transcription factor E4TF1-60) is encoded by the GABPA (Also known as E4TF1A) gene (Gene ID 2551) in human. Initially identified through their interactions with cytochrome c and cytochrome oxidase promoters, GABPA (NRF-2) and the related nuclear respiratory factor NRF-1 target many nuclear genes essential for mitochondrial respiratory function, including the mitochondrial transcription specificity factors TFB1M and TFB2M. GABPA plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. GABPA is upregulated in response to oxidant exposure or increased cellular reactive oxygen species (ROS), where it mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.
Immunogeno
Recombinant protein corresponding to human NRF2a/GABPA.
Applicazioni
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected GABPA (NRF-2alpha) occupancy at the TFB1M and TFB2M promoters by ChIP using HeLa chromatin preparations (Gleyzer, N., et al. (2005). Mol Cell Biol. 2005 Feb;25(4):1354-66).
Western Blotting Analysis: A representative lot detected GABPA (NRF-2alpha) in U2OS cell lysates (Gleyzer, N., and Scarpulla, R.C. (2011). J. Biol. Chem. 286(46):39715-39725; Gleyzer, N., and Scarpulla, R.C. (2013). J. Biol. Chem. 288(12):8004-8015).
Electrophoretic Mobility Shift Assay (EMSA) Analysis: A representative lot caused a supershift of GABPA (NRF-2alpha)-DNA complex in EMSA using recombinant GABPA or heparin agarose-purified HeLa nuclear extract and radiolabeled synthetic oligonucleotides corresponding to cytochrome oxidase subunit IV, hTFB1M, or hTFB2M promoter sequence with GABPA recognition sites (Gleyzer, N., et al. (2005). Mol Cell Biol. 2005 Feb;25(4):1354-66).
Electrophoretic Mobility Shift Assay (EMSA) Analysis: A representative lot caused a supershift of GABPA (NRF-2alpha)-DNA complex in EMSA using in vitro translated GABPA and radiolabeled cytochrome oxidase subunit IV promoter fragment containing tandem GABPA recognition sites (Vercauteren, K., et al. (2008). J Biol Chem. 283(18):12102-12111).
Immunoprecipitation Analysis: A representative lot immunoprecipitated GABPA (NRF-2alpha)-PRC complex from human embryonic kidney 293FT cell lysate (Vercauteren, K., et al. (2008). J Biol Chem. 283(18):12102-12111).
Western Blotting Analysis: A representative lot detected GABPA (NRF-2alpha) in U2OS cell lysates (Gleyzer, N., and Scarpulla, R.C. (2011). J. Biol. Chem. 286(46):39715-39725; Gleyzer, N., and Scarpulla, R.C. (2013). J. Biol. Chem. 288(12):8004-8015).
Electrophoretic Mobility Shift Assay (EMSA) Analysis: A representative lot caused a supershift of GABPA (NRF-2alpha)-DNA complex in EMSA using recombinant GABPA or heparin agarose-purified HeLa nuclear extract and radiolabeled synthetic oligonucleotides corresponding to cytochrome oxidase subunit IV, hTFB1M, or hTFB2M promoter sequence with GABPA recognition sites (Gleyzer, N., et al. (2005). Mol Cell Biol. 2005 Feb;25(4):1354-66).
Electrophoretic Mobility Shift Assay (EMSA) Analysis: A representative lot caused a supershift of GABPA (NRF-2alpha)-DNA complex in EMSA using in vitro translated GABPA and radiolabeled cytochrome oxidase subunit IV promoter fragment containing tandem GABPA recognition sites (Vercauteren, K., et al. (2008). J Biol Chem. 283(18):12102-12111).
Immunoprecipitation Analysis: A representative lot immunoprecipitated GABPA (NRF-2alpha)-PRC complex from human embryonic kidney 293FT cell lysate (Vercauteren, K., et al. (2008). J Biol Chem. 283(18):12102-12111).
This Anti-NRF2a/GABPA Antibody is validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP), Electrophoretic Mobility Shift Assay for the detection of NRF2a/GABPA.
Qualità
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected NRF2a/GABPA in 10 µg of HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected NRF2a/GABPA in 10 µg of HeLa cell lysate.
Descrizione del bersaglio
~55 kDa observed
Altre note
Concentration: Please refer to lot specific datasheet.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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