ABC242

Anti-CRKL Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Crk-like protein
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: rat, human, mouse
• Reattività contro le specie (prevista in base all’omologia): rhesus macaque (based on 100% sequence homology)
• tecniche: immunoprecipitation (IP): suitable; western blot: suitable
• N° accesso NCBI: NP_005198
• N° accesso UniProt: P46109
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CRKL(1399)

Descrizione

Descrizione generale

Crk-like protein (CRKL) is a 39 kDa adaptor protein with one SH2 and two SH3 binding domains. CRKL is the major protein which is tyrosine phosphorylated in response to BCR/ABL (chronic myelogenous leukemia) and growth factor activation. In myeloid cells, it binds to proteins such as paxillin, p130cas and p120 cbl. CRKL aslo appears to bind ABL and SOS.

Immunogeno

KLH-conjugated linear peptide corresponding to human CRKL.

Applicazioni

Detect Crk-like protein using this rabbit polyclonal antibody, Anti-CRKL Antibody validated for use in western blotting & IP.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 0.25 µg/mL from a representative lot detected CRKL in 10 µg of NIH3T3 and L6 cell lysate.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated CRKL in 500 µg of Jurkat cell lysate.

Qualità

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected CRKL in 10 µg of Jurkat cell lysate.

Descrizione del bersaglio

~39 kDa observed. Uncharacterized band(s) may appear in some lysates.

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable