ABC212

Anti-phospho ULK1 Antibody (Thr574)

from rabbit, purified by affinity chromatography

• Sinonimo/i: Serine/threonine-protein kinase ULK1, Serine/threonine-protein kinase Unc51.1, Unc-51-like kinase 1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)
• tecniche: inhibition assay: suitable (peptide); western blot: suitable
• N° accesso NCBI: NP_033495
• N° accesso UniProt: O70405
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pThr574)
• Informazioni sul gene: human ... ULK1(8408)

Descrizione

Descrizione generale

ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.

Immunogeno

KLH-conjugated linear peptide corresponding to mouse ULK1 phosphorylated at Thr574.

Applicazioni

Detect Autophagy-related protein;ATG1 using this rabbit polyclonal antibody, Anti-phospho ULK1 Antibody (Thr574) validated for use in western blotting & Peptide Inhibition Assay.

Western Blotting/Peptide Inhibition Analysis: 0.5 µg/mL from a representative detected phospho ULK1 in WB/PIA.

Qualità

Evaluated by Western Blotting in untreated and Calyculin A with okadaic acid treated Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho ULK1 (Thr574) in 10 µg of Jurkat cell lysate.

Descrizione del bersaglio

~120 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable