71772
ProteoExtract® Transmembrane Protein Extraction Kit
Sinonimo/i:
Membrane Protein Isolation Kit
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
41106500
NACRES:
NA.77
Prodotti consigliati
Produttore/marchio commerciale
Novagen®
Condizioni di stoccaggio
OK to freeze
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Descrizione generale
The ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).
Applicazioni
ProteoExtract® Transmembrane Protein Extraction Kit has been used: for extraction of protein from the apex segment of the colon in western blot analysis of Na+/H+ exchanger (NHE3) for extracting plasma membrane proteins
Caratteristiche e vantaggi
- No sonication, intensive vortexing, lengthy ultracentrifugation, or high-temperature incubation is needed.
- Reagents are sufficient to process a total of 40 samples.
Attenzione
Toxicity: Toxic (F)
Altre note
Nakamura, E., et al. 2008. Cancer Sci.99, 1390.
Note legali
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Skin Irrit. 2
Codice della classe di stoccaggio
10 - Combustible liquids
Certificati d'analisi (COA)
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