616430

TGF-α, Human, Recombinant, E. coli

• Sinonimo/i: TGF-α, Human, Recombinant, E. coli, Transforming Growth Factor-α
• Numero CAS: 105186-99-0
• Numero MDL: MFCD00082077
• Codice UNSPSC: 12352202

Proprietà

• Saggio: ≥97% (SDS-PAGE)
• Livello qualitativo: 100
• Stato: lyophilized
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Impurezze: ≤100 pg/μg Endotoxin (pg/μg TGF-α)
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

Recombinant, human transforming growth factor-α expressed in E. coli. Plays an important role in cell-cell adhesion and cell growth and differentiation. Reported to play a role in the development of hormonal insensitivity in estrogen-receptor-positive breast cancers.

Azioni biochim/fisiol

ED₅₀= 0.1-0.4 ng/ml as measured by the ability to stimulate ³H-thymidine incorporation in the mouse fibroblast cell line Balb/3T3

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

Lyophilized from 30% acetonitrile, 0.1% TFA.

Ricostituzione

Following reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml with sterile 10 mM acetic acid containing ≥0.1% HSA or BSA.

Altre note

Nicholson, R.I., et al. 1994. Cancer Res. 54, 1684.
Anklesaria, P., et al. 1990. Proc. Natl. Acad. Sci. USA87, 3289.
Massague, J. 1990. J. Biol. Chem.265, 21393.
Watanabe, S., et al. 1987. Proc. Natl. Acad. Sci. USA84, 1258.
Schreiber, A.B., et al. 1986. Science232, 1250.
Tam, J.P. 1985. Science229, 673.
Derynck, R., et al. 1984. Cell38, 327.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany