540020

Pluripotin

• Sinonimo/i: Pluripotin, Ras GTPase-Activating Protein Inhibitor II, SC1, N-(3-(7-(1,3-dimethyl-1H-pyrazol-5-ylamino)-1-methyl-2-oxo-1,2-dihydropyrimido[4,5-d]pyrimidin-3(4H)-yl)-4-methylphenyl)-3-(trifluoromethyl)benzamide, ERK Inhibitor VII
• Formula empirica (notazione di Hill): C₂₇H₂₅F₃N₈O₂
• Peso molecolare: 550.54
• Codice UNSPSC: 12352200

Proprietà

• Saggio: ≥97% (HPLC)
• Stato: solid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; protect from light
• Colore: yellow
• Solubilità: DMSO: 50 mg/mL
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

A cell-permeable dihydropyrimido-pyrimidine compound that effectively upregulates GTP-bound active Ras level and inhibits LIF- (leukemia inhibitory factor) induced Erk1/2 phosphorylation in OG2-mES (murine Embryonic Stem) cells (1 µM) via direct interaction with Erk and RasGAP (K_D = 212 and 98 nM in binding RasGAP 729-1042 catalytic domain and Erk1, respectively), without affecting cellular STAT3 or GSK-3β/Wnt signaling. Pluripotin/SC1 is shown to greatly complement and enhance the efficiency of LIF in deriving ESCs from blastocysts of several murine strains and effectively maintain the pluripotent state of the ESCs even after 11 passages in cultures. SC1-expanded ESCs effectively form EBs (embryonic bodies) in vitro upon SC1 and LIF withdrawal and are capable of generating chimeric offsprings from surrogate mice when injected into blastocysts from different strains in vivo.

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Standard Handling (A)

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Altre note

Yang, W., et al. 2009. Stem Cells27, 383.
Chen, S., et al. 2006. Proc. Natl. Acad. Sci. USA103, 17266.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable