454854
MAP Kinase 2, His•Tag®, Human, Recombinant, E. coli
Sinonimo/i:
ERK2, MAPK2, p42 MAPK, ERK2, MAPK2, p42MAPK
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About This Item
Codice UNSPSC:
12352202
Ricombinante
expressed in E. coli
Livello qualitativo
Saggio
≥95% (SDS-PAGE)
Forma fisica
liquid
Attività specifica
≥5500 units/mg protein
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
avoid repeated freeze/thaw cycles
Attività estranea
Proteases, none detected
Condizioni di spedizione
wet ice
Temperatura di conservazione
−70°C
Descrizione generale
Recombinant, human MAP Kinase 2 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
Recombinant, human MAP Kinase 2 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
Attenzione
Toxicity: Standard Handling (A)
Definizione di unità
One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
Stato fisico
In 150 mM NaCl, 50 mM Tris, 1 mM DTT, 50% glycerol, pH 8.5.
Ricostituzione
Following initial thaw, aliquot and freeze (-70°C).
Altre note
Lew, J. 2003. Biochemistry42, 849.
Johnson, G.L., and Lapadat, R. 2002. Science298, 1911.
Koistinaho, M., and Koistinaho, J. 2002. Glia40, 175.
Johnson, G.L., and Lapadat, R. 2002. Science298, 1911.
Koistinaho, M., and Koistinaho, J. 2002. Glia40, 175.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Milla Koistinaho et al.
Glia, 40(2), 175-183 (2002-10-16)
Although microglial cells are thought to play a beneficial role in the regeneration and plasticity of the central nervous system (CNS), recent studies have indicated that at least some molecules released by microglia may be harmful in acute brain insults
Gary L Johnson et al.
Science (New York, N.Y.), 298(5600), 1911-1912 (2002-12-10)
Multicellular organisms have three well-characterized subfamilies of mitogen-activated protein kinases (MAPKs) that control a vast array of physiological processes. These enzymes are regulated by a characteristic phosphorelay system in which a series of three protein kinases phosphorylate and activate one
John Lew
Biochemistry, 42(4), 849-856 (2003-01-29)
Protein kinases constitute one of the largest enzyme families encoded by the human genome. Owing to their critical role in virtually all aspects of signal transduction, protein kinases have evolved stringent mechanisms for their regulation, which classically falls into two
Single-Molecule Force Spectroscopy on the N2A Element of Titin: Effects of Phosphorylation and CARP.
Thomas Lanzicher et al.
Frontiers in physiology, 11, 173-173 (2020-04-08)
Titin is a large filamentous protein that forms a sarcomeric myofilament with a molecular spring region that develops force in stretched sarcomeres. The molecular spring has a complex make-up that includes the N2A element. This element largely consists of a
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