407143-M

Anti-IP₃ Receptor Rabbit pAb

liquid, Calbiochem^®

• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: liquid
• contiene: ≤0.1% sodium azide as preservative
• Reattività contro le specie: human, canine, mouse, rat, porcine, bovine
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• Isotipo: IgG
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ITPR1(3708) ; mouse ... Itpr1(16438) ; rat ... Itpr1(25262)

Descrizione

Descrizione generale

Rabbit polyclonal antibody purified by ammonium sulfate precipitation and ion-exchange (DEAE) column chromatography. Recognizes the ~260 kDa IP₃ receptor protein.

Recognizes all of the ~260 kDa IP₃ receptor subtypes.

This Anti-IP₃ Receptor Rabbit pAb is validated for use in ELISA, FC, Immunoblotting, IF, IHC, IP, Radioimmunoassay for the detection of IP₃ Receptor.

Immunogeno

a synthetic peptide corresponding to amino acids in the C-terminal cytoplasmic domain of the IP₃ receptor

Applicazioni

ELISA (1:2000)

Flow Cytometry (1:1000)

Immunoblotting (1:250)

Immunofluorescence (1:200)

Immunohistochemistry (1:200)

Immunoprecipitation (1:200)

Radioimmunoassay (1:10000)

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.

Ricostituzione

Following initial thaw, aliquot and freeze (-20°C).

Altre note

Bourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.

Variables associated with assay conditions will dictate the proper working dilution.



Recommended Protocol for Immunoblotting using anti-IP₃ Receptor Antibodies



NOTE: For protein loading, we recommend 1 x 10⁶ cells/gel lane or 100 μg/gel lane.



• TBST

• 20 mM Tris-HCl, pH 7.6

• 150 mM NaCl

• 0.05% Tween^®-20 detergent



1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody).

2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST.

3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h.

4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.

5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP.

6. Shake for 1 h.

7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.

8. Mix development solution (1:1) (Amersham ECL kit).

9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable