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Merck

1.50798

Purospher® RP-18 endcapped (5 µm) HPLC Columns

L × I.D. 125 mm x 3 mm LiChroCART HPLC cartridge, manuCART holder required for use

Sinonimo/i:

Purospher® RP-18 endcapped (5 µm) LiChroCART 125-3

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Informazioni su questo articolo

UNSPSC Code:
41115709
NACRES:
SB.52
Particle size:
5 μm
Matrix active group:
C18 (RP18), C18 bonding phase
Pore size:
120 Å pore size
Matrix:
fully porous particle
Servizio Tecnico
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Nome del prodotto

Purospher® RP-18 endcapped (5 µm), L × I.D. 125 mm x 3 mm LiChroCART HPLC cartridge, manuCART holder required for use

material

stainless steel column

Quality Segment

agency

suitable for USP L1

product line

Purospher®

feature

endcapped

packaging

1 ea of

extent of labeling

18% Carbon loading

parameter

250 bar pressure, 65 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

column L × I.D.

12.5 cm × 3 mm

surface area

350 m2/g

matrix

fully porous particle

matrix active group

C18 (RP18), C18 bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH

2-7.5

separation technique

reversed phase

storage temp.

no temp limit

General description

HPLC cartridge
Purospher® RP-18 endcapped is a versatile high-purity HPLC column producing excellent peak shapes. The excellent balance of Purospher<TMSYMBOL></TMSYMBOL> RP-18 endcapped in the sum of its chromatographic properties is the key to better separations of complex samples with simpler, neutral eluents. The good chemical stability of Purospher<TMSYMBOL></TMSYMBOL> RP-18 endcapped enables the use of mobile phase conditions at pH 8 for a long period of time. The connection to the HPLC system is being accomplished by use of the manu-CART NT cartrige holder (1.51486).

Application


  • Determination of nifedipine in serum of women in preterm labor by high-performance liquid chromatography with diode array detection.: This study utilized Purospher® RP-18 endcapped (5 µm) columns in the HPLC analysis of nifedipine levels in serum samples from women in preterm labor. The research demonstrated the effectiveness of the Purospher® RP-18 columns in achieving high resolution and sensitivity, making it a valuable tool for bioanalytical applications in clinical chemistry (Kowalczuk et al., 2006).

Analysis Note

Pressure: ≤ 60 bar
Theoretical Plates (N/m) (Anthracene): ≥ 65000
Symmetry (Anthracene): 0.8 - 1.4
Capacity factor (Anthracene): 4.5 - 6.5

Legal Information

LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany


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