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Merck

1.50255

Purospher® STAR RP-18 endcapped (5 µm) HPLC Columns

L × I.D. 125 mm x 2 mm LiChroCART HPLC cartridge, manuCART holder required for use

Sinonimo/i:

Purospher® STAR RP-18 endcapped (5 µm) LiChroCART 125-2

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Informazioni su questo articolo

UNSPSC Code:
41115709
NACRES:
SB.52
Particle size:
5 μm
Matrix active group:
C18 (RP18), C18 bonding phase
Pore size:
120 Å pore size
Matrix:
fully porous particle
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Nome del prodotto

Purospher® STAR RP-18 endcapped (5 µm), L × I.D. 125 mm x 2 mm LiChroCART HPLC cartridge, manuCART holder required for use

material

stainless steel column

Quality Segment

agency

suitable for USP L1

product line

Purospher®

feature

endcapped

packaging

1 ea of

extent of labeling

17% Carbon loading

parameter

250 bar pressure, 65 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

column L × I.D.

12.5 cm × 2 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

C18 (RP18), C18 bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

reversed phase

storage temp.

no temp limit

General description

Purospher® STAR RP-18 endcapped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher® STAR RP-18 endcapped, 5 µm HPLC columns allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase.Purospher® STAR RP-18 endcapped, 5 µm LiChroCART 125-2 is a cartridge column which requires a manuCART cartridge column holder part number 1.51486.0001. The manuCART can be used to hold one cartridge column with or without direct integration of a guard column. LiChroCART cartridge columns mounted with a manuCART cartridge column holder are hand-tight only and have therefore a maximum pressure stability of 250 bar. For higher pressure stability requirements Purospher® STAR RP-18 endcapped, 5 µm Hibar® RT 125-2 HPLC columns are available part number 1.50596.0001.The column dimension of 125 mm length x 2 mm I.D. is the optimal choice for HPLC separation of complex samples at lower flow rate, allowing significant solvent savings and improved sensitivityLearn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 65 bar
Theoretical Plates (N/m) (hexylbenzene): ≥ 70000
Symmetry (hexylbenzene): 0.8 - 1.4
Capacity factor (hexylbenzene): ≥ 8.0

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany


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