07-2129

Anti-phospho-Abi-1 (Ser225) Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Abl interactor 1, Abelson interactor 1, Abi-1, Abl-binding protein 4, AblBP4, Eps8 SH3 domain-binding protein, Eps8-binding protein, Nap1-binding protein, Nap1BP, Spectrin SH3 domain-binding protein 1, e3B1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: monkey, human
• Reattività contro le specie (prevista in base all’omologia): rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• tecniche: western blot: suitable
• N° accesso NCBI: NP_001012768
• N° accesso UniProt: Q8IZP0
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pSer225)
• Informazioni sul gene: human ... ABI1(10006)

Descrizione

Descrizione generale

Abl interactor 1 (Abelson interactor 1) is a member of the family of Abl-interactors and is highly expressed in the brain. Abl interactor 1 is involved in the signaling of ABL1 and ABL2 nonreceptor tyrosine kinases, and the EGF-receptor. The Abl interactor 1 protein contains SH3 domains, and may function as an adaptor protein; It forms a trimeric complex with EPS8 and SOS1 in the transduction of signals from Ras to Rac. Previous studies have suggested that Abl interactor 1 via the Ras and Rac pathways may regulate cell proliferation, cell adhesion, and actin remodeling. Abl interactor 1 is also investigated for its role in tumor suppression.

Specificità

This antibody recognizes Abi-1 phosphorylated at Ser225.

Immunogeno

Epitope: Phosphorylated Ser225

KLH-conjugated linear peptide corresponding to human Abi-1 phosphorylated at Ser225.

Applicazioni

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

This phospho-Abi-1 antibody is validated for use in WB for the detection of the phospho-Abi-1 protein.

Western Blot Analysis: A representative lot was used by an independent laboratory in human mammary cells treated with EGF and Abi-1-transfected 293T cells (Mendoza, M., et al. (2011). Molecular Cell 41:661–671).

Qualità

Evaluated by Western Blot in EGF-treated COS cells untreated and treated with lambda phosphatase.

Western Blot Analysis: 1 µg/mL of this antibody detected Abi-1 in 10 µg of EGF-treated COS cells untreated and treated with lambda phosphatase.

Descrizione del bersaglio

~60 kDa observed.
55 kDa calculated.

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
EGF-treated COS cells untreated and treated with lambda phosphatase.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable