07-1799

Anti-NCoA-2 Antibody

from rabbit

• Sinonimo/i: Transcriptional intermediary factor 2, glucocorticoid receptor-interacting protein-1, nuclear receptor coactivator 2, glucocorticoid receptor interacting protein 1, steroid receptor coactivator-2, transcriptional mediators/intermediary factor 2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: human
• tecniche: ChIP: suitable; western blot: suitable
• Isotipo: IgG
• N° accesso NCBI: NP_006531.1
• N° accesso UniProt: Q15596
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... NCOA2(10499)

Descrizione

Descrizione generale

NCoA2 (nuclear receptor coactivator 2) is a transcriptional coactivator for steroid receptors and nuclear receptors. Also known as glucocorticoid receptor interacting protein 1 (GRIP1), steroid receptor coactivator-2 (SRC-2), or transcriptional mediators/intermediary factor 2 (TIF2), this coregulatory protein contains several nuclear receptor interacting domains and an intrinsic histone acetyltransferase activity. NCoA-2 assists nuclear receptors in the upregulation of DNA expression. In cells ligand activated nuclear receptors recruit NCoA-2 to DNA where it acylates histones to make targeted regions accessible to transcriptional machinery.

Specificità

Homology to mouse and rat is 95%.

This antibody recognizes NCoA-2.

Immunogeno

Epitope: Unknown

Linear peptide corresponding to human NCoA-2

Applicazioni

Chromatin Immunoprecipitation:
A representative lot of this antibody was used for chIP. See Monroe, D.G., et al. (2006) J. Biol. Chem. 281(39):28596-28604.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-NCoA-2 Antibody (rabbit polyclonal antibody) validated in WB to detect NCoA-2 also known as Transcriptional intermediary factor 2, glucocorticoid receptor-interacting protein-1, nuclear receptor coactivator 2.

Qualità

Evaluated by Western Blot in U2 OS cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected SRC2 on 10 µg of U2 OS cell lysate.

Descrizione del bersaglio

~ 160 kDa

Stato fisico

Affinity purified

Format: Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
U2 OS cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable