07-1229
Anti-DAPK2 Antibody
from rabbit, purified by affinity chromatography
Sinonimo/i:
DAP kinase, DAP- kinase-related protein, Death-associated protein kinase
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
affinity purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Purificato mediante
affinity chromatography
Reattività contro le specie
rat, mouse, human
tecniche
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotipo
IgG
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... DAPK2(23604)
rat ... Dapk2(300799)
Descrizione generale
Death Associated Protein Kinase (DAPK) is a Ca2+/Calmodulin-dependent, cytoskeletal associated protein kinase whose expression is implicated in the sensitivity of cells to apoptotic effects of TNFα and Interferon-γ. Deletion of the Calmodulin-binding domain results in a constitutively active mutant with a more potent cytotoxic effect. In contrast, the catalytically inactive mutant exerts a dominant negative effect, reducing cytotoxicity and protecting cells from Interferon-γ induced cell death. DAPK expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlate with high rates of metastasis.
Specificità
Detects a 42 kDa band by western blot, corresponding to DAPK2. Does not cross-react with DAPK1.
Immunogeno
Peptide corresponding to aa 356-370 (ARRKALHPRRRSSTS) of human DAPK2, which is identical to the sequence of mouse origin.
Applicazioni
Detect DAPK2 using this Anti-DAPK2 Antibody validated for use in WB, IH(P).
Immunohistochemisty (paraffin): DAPK2 representative staining pattern/morphology in apoptotic bodies in colon cancer. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB.
Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
Research Category
Signaling
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
GPCR, cAMP/cGMP & Calcium Signaling
Qualità
Routinely evaluated by Western Blot on C2C12 lysates.
Western Blot Analysis:
1:500-1,1000 dilution of this lot detected DAPK2 on 10 μg of C2C12 lysates.
Western Blot Analysis:
1:500-1,1000 dilution of this lot detected DAPK2 on 10 μg of C2C12 lysates.
Descrizione del bersaglio
Detects a 42 kDa band by western blot.
Linkage
Replaces: AB3606
Stato fisico
Affinity purified
Purified rabbit polyclonal IgG in buffer containing PBS and 0.02% sodium azide.
Stoccaggio e stabilità
Stable for 6 months at 2-8° C from date of receiept.
Risultati analitici
Control
C2C12 cell lysate
C2C12 cell lysate
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Snehal Dabir et al.
International journal of cancer, 134(5), 1045-1054 (2013-08-21)
Protein inhibitor of activated signal transducer and activator of transcription 3 (STAT3) (PIAS3) is an endogenous inhibitor of STAT3 that negatively regulates STAT3 transcriptional activity and cell growth and demonstrates limited expression in the majority of human squamous cell carcinomas
B Inbal et al.
Molecular and cellular biology, 20(3), 1044-1054 (2000-01-11)
In this study we describe the identification and structure-function analysis of a novel death-associated protein (DAP) kinase-related protein, DRP-1. DRP-1 is a 42-kDa Ca(2+)/calmodulin (CaM)-regulated serine threonine kinase which shows high degree of homology to DAP kinase. The region of
Lidia Villanova et al.
International journal of molecular sciences, 21(7) (2020-04-05)
MicroRNAs are tiny but powerful regulators of gene expression at the post-transcriptional level. Aberrant expression of oncogenic and tumor-suppressor microRNAs has been recognized as a common feature of human cancers. Colorectal cancer represents a major clinical challenge in the developed
T Kawai et al.
Oncogene, 18(23), 3471-3480 (1999-06-22)
We have identified and characterized a new calcium/calmodulin (Ca2+/CaM) dependent protein kinase termed death-associated protein kinase 2 (DAPK2) that contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.