06-1130
Anti-RNF168 Antibody
Sinonimo/i:
E3 ubiquitin-protein ligase RNF168, RING finger protein 168
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
Descrizione generale
RNF168 is a ubiquitin ligand that binds to ubiquinated histone H2A and localizes to sites of DNA damage. RNF168-dependent chromatin modifications cause 53BP1 and BRCA1 to accumulated at the site. A mutation in RNF168 has been associated with RIDDLE syndrome, an immunodeficiency and radiosensitivity disorder. RNF168 is responsible for securing repair factors at sites of DNA damage. RNF168 has two MIU (motif interacting with ubiquitin) and one UMI (UIM- and MIU-related UBD) ubiquitin binding domains (UBDs) which are essential for proper localization of RNF168.
Specificità
This antibody recognizes RNF168 at the C-terminus.
Immunogeno
Histidine-tagged recombinant protein corresponding to the C-terminus of human RNF168.
Applicazioni
Anti-RNF168 Antibody is a Rabbit Polyclonal Antibody for detection of RNF168 also known as E3 ubiquitin-protein ligase RNF168 & has been validated in WB.
Immunoprecipitation Analysis: A representative lot of this antibody was used to immunoprecipitate RNF168 from whole cell lysates (Zhao, H., et al. (2014) Cell Cycle. 13(11):1–11).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Qualità
Evaluated by Western Blot in Jurkat cell lysate.
Western Blot Analysis: 0.1 μg/mL of this antibody detected RNF168 on 10 μg of Jurkat cell lysate.
Western Blot Analysis: 0.1 μg/mL of this antibody detected RNF168 on 10 μg of Jurkat cell lysate.
Descrizione del bersaglio
~79 kDa observed.
Stato fisico
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Risultati analitici
Control
Jurkat cell lysate
Jurkat cell lysate
Altre note
Concentration:Please refer to the Certificate of Analysis for the lot-specific concentration.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
Codice della classe di stoccaggio
10-13 - German Storage Class 10 to 13
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Hongchang Zhao et al.
Cell cycle (Georgetown, Tex.), 13(11), 1777-1787 (2014-04-16)
Timely and proper cellular response to DNA damage is essential for maintenance of genome stability and integrity. B-cell lymphoma/leukemia 10 (BCL10) facilitates ubiquitination of NEMO in the cytosol, activating NFκB signaling. Translocation and/or point mutations of BCL10 associate with mucosa-associated
Katherine N Choe et al.
EMBO reports, 17(6), 874-886 (2016-05-06)
Defects in DNA replication, DNA damage response, and DNA repair compromise genomic stability and promote cancer development. In particular, unrepaired DNA lesions can arrest the progression of the DNA replication machinery during S-phase, causing replication stress, mutations, and DNA breaks.
Kathy Shire et al.
Journal of cell science, 129(3), 580-591 (2015-12-18)
Promyelocytic leukemia (PML) protein forms the basis of PML nuclear bodies (PML NBs), which control many important processes. We have screened an shRNA library targeting ubiquitin pathway proteins for effects on PML NBs, and identified RNF8 and RNF168 DNA-damage response
Tina Gruosso et al.
EMBO molecular medicine, 8(5), 527-549 (2016-03-24)
Anti-cancer drugs often increase reactive oxygen species (ROS) and cause DNA damage. Here, we highlight a new cross talk between chronic oxidative stress and the histone variant H2AX, a key player in DNA repair. We observe that persistent accumulation of
Meilen C Muñoz et al.
Nucleic acids research, 42(12), 7720-7733 (2014-05-16)
The E3 ubiquitin ligase RNF168 is a DNA damage response (DDR) factor that promotes monoubiquitination of H2A/H2AX at K13/15, facilitates recruitment of other DDR factors (e.g. 53BP1) to DNA damage, and inhibits homologous recombination (HR) in cells deficient in the
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