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Documenti fondamentali

05-1579

Sigma-Aldrich

Anti-Axin-1 Antibody, clone A5

clone A5, from mouse

Sinonimo/i:

Axis inhibition protein 1, axin 1, axis inhibitor 1, fused, mouse, homolog of

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100 μG
CHF 388.00

CHF 388.00


Spedizione prevista il28 maggio 2025


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Cambia visualizzazione
100 μG
CHF 388.00

About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CHF 388.00


Spedizione prevista il28 maggio 2025


Richiedi un ordine bulk

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

A5, monoclonal

Reattività contro le specie

human

Reattività contro le specie (prevista in base all’omologia)

mouse (based on 100% sequence homology)

tecniche

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG2aκ

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... AXIN1(8312)
mouse ... Axin1(12005)

Descrizione generale

Axin-1 is constitutively expressed and acts as a negative regulator of Wnt expression. It has been demonstrated that mouse Wnt, Wnt3a, is secreted by multiple cell lines and can act and as a growth factor for stem cells. Secreted Wnt3a is highly hydrophobic because it is palmitoylated on an essential cysteine C77; the palmitoylated state of Wnt3a is essential for stem cell signaling. Incubation of stem cells with Wnt3a in tissue culture medium resulted in the increased number of mouse hematopoietic stem cells expressing "primitive" phenotype.

Specificità

This antibody recognizes the N-terminus of Axin-1.

Immunogeno

GST-tagged recombinant protein corresponding to the N-terminus of mouse Axin-1.

Applicazioni

Detect Axin-1 using this Anti-Axin-1 Antibody, clone A5 validated for use in WB, IP & IC.

Qualità

Evaluated by Western Blot using immunoprecipitated Axin-1 in SW-480 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Axin-1 immunoprecipitated from 10 µg of SW-480 cell lysate.

Descrizione del bersaglio

~ 97 kDa

Stato fisico

Format: Purified

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Chuifeng Fan et al.
Molecular carcinogenesis, 54 Suppl 1, E138-E147 (2014-09-30)
Our previous studies indicate that abnormal expression of several Wnt signaling molecules including Axin, Dvl and β-catenin are involved in proliferation, invasion and metastasis of lung cancer. Zbed3 was found to inhibit function of Axin-GSK3β complex and thus lead to
Phosphatidylinositol 3-kinase signaling does not activate the wnt cascade.
Ng, SS; Mahmoudi, T; Danenberg, E; Bejaoui, I; de Lau, W; Korswagen, HC; Schutte, M; Clevers, H
The Journal of Biological Chemistry null
S Monteagudo et al.
Osteoarthritis and cartilage, 30(5), 724-734 (2022-03-02)
To investigate how ANP32A, previously linked to the antioxidant response, regulates Wnt signaling as unraveled by transcriptome analysis of Anp32a-deficient mouse articular cartilage, and its implications for osteoarthritis (OA) and diseases beyond the joint. Anp32a knockdown chondrogenic ATDC5 cells were
Rong Xie et al.
eLife, 11 (2022-12-22)
Axin1 is a key regulator of canonical Wnt signaling pathway. Roles of Axin1 in skeletal development and in disease occurrence have not been fully defined. Here, we report that Axin1 is essential for lower limb development. Specific deletion of Axin1
Eunsun Kim et al.
Nature communications, 10(1), 1909-1909 (2019-04-25)
Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related deaths worldwide. β-catenin is widely thought to be a major oncogene in HCC based on the frequency of mutations associated with aberrant Wnt signaling in HCC patients. Challenging this model

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